在Cd应力下通过基因素乙化修饰通过pakchoi中反应性氧分子的调节
Xiaoqun Cao1, Ming Zhang2, Xufeng Xiao1
1College of Agronomy, Jiangxi Agricultural University, Nanchang, Jiangxi, P. R. China.
PloS one
|November 20, 2024
概括
应激会增加活性氧物种 (ROS) 和损伤细胞. 表观遗传修饰抑制剂,特别是低剂量,通过增强激素乙化来减轻这些影响,为应激反应提供了新的见解.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 环境毒理学环境毒理学
背景情况:
- 反应性氧物种 (ROS) 在非生物压力期间在表观遗传修饰中发挥作用.
- 在 (Cd) 压力下ROS和表观遗传调节之间的相互作用尚未得到充分理解.
研究的目的:
- 调查帕克乔伊幼苗在Cd压力下的ROS和表观遗传修饰之间的相互调节机制.
- 探索表观遗传修饰抑制剂在缓解Cd诱导的细胞损伤方面的潜力.
主要方法:
- 受到Cd压力的帕科伊苗木接受了六种表观遗传修饰抑制剂 (5-AC,RG108,TSA,CUDC101,AT13148,H89) 的治疗.
- 评估了ROS积累,染色体脱凝,细胞循环停止和DNA损伤.
- 分析了基因组乙化水平和基因组乙转移酶和脱乙转移酶基因的表达.
- 评估了一种抗氧化剂 (Thi) 和不同度的TSA的影响.
主要成果:
- Cd压力导致ROS积累和染色质脱凝.
- 低度的表观遗传抑制剂增加了基因素乙化,减弱了Cd诱导的ROS损伤,细胞循环停止和DNA损伤.
- 基因素乙化水平由基因素乙转移酶和脱乙转移酶基因转录共同调节.
- 抗氧化剂Thi增强了抑制剂的保护作用.
- 高的TSA度加剧了Cd诱导的ROS积累.
结论:
- 表观遗传调节,特别是染色质修饰,与Cd压力下的ROS通路有关.
- 染色体修饰可能通过调节基因表达来影响ROS积累.
- 这项研究为表观遗传修饰的调节机制提供了新的视角,以应对非生物压力.
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