在Litchi chinensis中,在冷却诱导的开花过程中,基斯改性H3K27me3是必不可少的
Xifen Pan1, Xingyu Lu1,2, Lijie Huang1,3
1Guangdong Litchi Engineering Research Center, College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
Plant physiology
|November 20, 2024
概括
通过基因素H3 lysine 27三甲基化 (H3K27me3) 的表观遗传调节对于化诱导的花期至关重要. 抑制H3K27me3沉积延迟了开花,揭示了它在花诱导中的作用.
科学领域:
- 植物生物学 植物生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
背景情况:
- 花 (Litchi chinensis) 的开花是由低温引发的.
- 在调节植物发育方面,表观遗传机制,特别是基因组修饰,越来越被认可.
研究的目的:
- 调查素H3素27三甲基化 (H3K27me3) 在化诱导的花诱导 (FId) 中的作用.
- 在花过渡期间识别受H3K27me3调节的基因.
主要方法:
- 基因组分析H3K27me3在不同发育阶段的沉积 (花前诱导,花诱导,花开始).
- 整合RNA-seq和ChIP-seq数据以识别差异调节基因 (DRG).
- 使用GSK343和基因表达分析进行H3K27me3的药理抑制.
- 基因协同表达网络分析和Arabidopsis thaliana的异质表达.
主要成果:
- 在李子芽和叶子中鉴定出数千个差异化H3K27me3修饰基因 (DHG) 和差异调节基因 (DRG).
- 通过GSK343在LcTPS1和LcFRI位点抑制H3K27me3,导致基因表达增加,花期延迟和花期减少.
- LcSVP10被确定为开花时间的关键调节器,在Arabidopsis中保留了功能.
结论:
- H3K27me3在子中冷却诱导的开花的表观遗传调节中发挥着关键作用.
- 表观遗传修饰为园艺作物中调节开花时间提供了潜在的目标.
- 了解这些机制可以有助于改进的种植和育种策略.
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