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转录组分析揭示了大鱼 (Micropterus salmoides) 对盐度适应的分子反应

Yichun Zhang¹, Jinxin Zhang², Yafang Tan¹

¹Collaborative Innovation Center of Nanfan and High-Efficiency Tropical Agriculture, Hainan University; State Key Laboratory of Marine Resource Utilization in South China Sea; Hainan Aquaculture Breeding Engineering Research Center; Hainan Academician Team Innovation Center; Sanya Nanfan Research Institute of Hainan University; School of Marine Biology and Fisheries, Hainan University, Haikou 570228, China.

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概括

这项研究揭示了大鱼脏如何通过调节排毒基因来适应高盐度,从而帮助脏受伤的恢复,并支持水产养殖.

科学领域:

*水生生理学和分子生物学
* 环境适应机制

背景情况:

*土地化和全球变暖的增加需要了解鱼类适应度升高的情况.
* 作为一个关键的水产养殖物种,Micropterus salmoides (大鱼) 的脏中的盐度耐受机制尚不清楚.

研究的目的:

* 为了研究Micropterus salmoides脏中盐度耐受性的分子机制.
* 确定差异表达基因 (DEGs) 和关键途径,参与适应不同盐度水平.

主要方法:

*RNA测序 (RNA-seq) 用于分析M. salmoides脏中的基因表达,度为0‰,5‰和10‰,分别为24天和48天.
*进行了差异基因表达分析和途径丰富分析.

主要成果:

* DEGs主要在代谢 (二次代谢物生物合成,酸代谢) 和免疫 (IL-17信号传递,ECM受体相互作用) 途径中得到丰富.
* 细胞染色体P450异生物代谢途径中盐度上调的基因增加,包括UDP-glucuronosyltransferases (UGTs) 和谷氨S转移酶 (GSTs).
* 低调基因与细胞周期和胞体通路有关; 囊A1显著上调,表明损伤恢复.

结论:

*Micropterus salmoides脏可能通过调节UGTs和GSTs来减轻盐度诱导的有毒物质和氧化损伤.
*像Cystatin A1这样的基因的升级支持度压力下脏损伤的恢复.
*研究结果提供了M. salmoides对高盐度的分子适应能力的见解,并为耐盐度菌株的育种提供了信息.

关键词:

脏 - 脏 - 脏微型鱼类 (Micropterus salmoides) 是一种鱼类.盐分盐分盐分盐分盐分转录组转录组就是一个转录组.

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