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Amyloid Fibrils

Amyloid Fibrils

Amyloid fibrils are aggregates of misfolded proteins. Under most circumstances, misfolded proteins are either refolded by chaperone proteins or degraded by the proteasome. However, in the case of a mutation or a disease, these proteins can accumulate to form large clusters and often further assemble to form elongated fibers, called fibrils.
Amyloid deposits were observed as early as 1639 in the liver and the spleen. In 1854, Rudolph Virchow performed iodine staining,...

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Updated: Jun 7, 2025

Characterization of pH-Dependent Reversible Self-Assembly of Amyloid Beta 1-40-Coated Gold Colloids

Characterization of pH-Dependent Reversible Self-Assembly of Amyloid Beta 1-40-Coated Gold Colloids

Published on: March 21, 2025

描述粉样蛋白形成过程中的异质性.

Hoi Sung Chung¹

¹Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, 20892-0520, USA.

Current opinion in structural biology

|November 20, 2024

概括

了解蛋白质聚合,这是疾病的关键因素,需要描述其复杂的途径. 新的方法正在改善我们对粉样蛋白形成及其中间体的理解.

科学领域:

生物化学和分子生物学
结构生物学结构生物学
神经科学是一个神经科学.

背景情况:

蛋白质聚合涉及从单体到粉样纤维的众多途径.
纤维状多态体的原子分辨率结构是已知的,但像寡合体这样的中间阶段是未知的.
描述聚合异质性对于理解疾病机制和开发治疗方法至关重要.

研究的目的:

审查最近在粉样蛋白形成过程中表征异质性的进展.
突出研究中间聚合状态的重要性.

主要方法:

审查结构确定技术的最新发展.
专注于适用于异质蛋白质聚合过程的方法.
在结构研究中讨论固态NMR和冷电子显微镜 (cryoEM).

主要成果:

固态NMR和冷EM等技术的进步使得粉样纤维的原子层结构确定成为可能.
在理解中间物种 (如寡合物) 的异质性质方面仍然存在重大差距.
该综述综合了当前关于表征这些复杂聚合途径的知识.

结论:

精确地描述蛋白质聚合中的异质性至关重要.

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Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain

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Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging

Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging

Published on: October 20, 2017

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Last Updated: Jun 7, 2025

Characterization of pH-Dependent Reversible Self-Assembly of Amyloid Beta 1-40-Coated Gold Colloids

Characterization of pH-Dependent Reversible Self-Assembly of Amyloid Beta 1-40-Coated Gold Colloids

Published on: March 21, 2025

Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain

Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain

Published on: April 28, 2022

Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging

Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging

Published on: October 20, 2017

为了全面理解,需要对中间物种进行进一步的研究.

阐明这些过程是确定粉样蛋白相关疾病的治疗点的关键.