寒冷诱导的热生成需要在棕色脂肪细胞中通过中性脂酶介导的细胞内脂解
Etienne Mouisel1, Anaïs Bodon1, Christophe Noll2
1Institut des Maladies Métaboliques et Cardiovasculaires, I2MC, Université de Toulouse, Inserm, Université Toulouse III-Paul Sabatier (UPS), Toulouse, France.
Cell metabolism
|November 20, 2024
概括
由脂肪三糖 lipase (ATGL) 和激素敏感 lipase (HSL) 进行的细胞内脂解对于棕色脂肪组织 (BAT) 热生成和在暴露在寒冷中保持体温至关重要.
科学领域:
- 代谢过程中的代谢.
- 热生成是一种热生成.
- 脂肪组织生物学 脂肪组织生物学
背景情况:
- 长链脂肪酸是棕色脂肪组织 (BAT) 热生成的关键燃料.
- 之前的研究质疑棕色脂肪细胞细胞内脂解在冷引起的热生成中的作用.
研究的目的:
- 调查脂肪三糖 lipase (ATGL) 和激素敏感 lipase (HSL) 在BAT热生成中的功能.
- 为了确定棕色脂肪细胞细胞内脂解的必要性,冷诱导的非热生成.
主要方法:
- 为ATGL和HSL (BAHKO) 生成可诱导的棕色脂肪细胞特异性淘汰小鼠.
- 评估了BAT形态,线粒体含量和体温调节在寒冷暴露期间.
- 使用[11C]-乙酸盐和[11C]-棕酸盐的正子发射断层扫描计算断层扫描 (PET-CT) 来评估BAT氧化活性和脂肪酸代谢.
主要成果:
- BAHKO小鼠表现出过度化的棕色脂肪与扩大的脂质滴,但保留了线粒体.
- 在禁食的BAHKO小鼠中,感冒诱导的热生成和体温维持受损.
- 在BAHKO小鼠中,PET-CT揭示了废弃的冷诱导的BAT氧化活性和受损的脂肪酸代谢.
结论:
- 在棕色脂肪细胞中由ATGL和HSL介导的细胞内脂解对于BAT热生成是不可或缺的.
- 这条通路对于保持体温至关重要,特别是在冷暴露期间的禁食条件下.
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