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血清细胞外囊泡中的细菌信息反映了附着周围脂肪的炎症

Toshihiro Uemura¹, Atsunari Kawashima¹, Kentaro Jingushi²

¹Department of Urology, Graduate School of Medicine, Osaka University, Suita, Japan.

|November 20, 2024

概括

细胞外囊泡 (EVs) 中的细菌DNA可能表明脂肪组织炎症. 使用特定细菌DNA的EPC指数与细胞癌患者的炎症风险降低相关.

科学领域:

微生物学微生物学
代谢学代谢学代谢学
在瘤学瘤学.

背景情况:

脂肪组织和肠道微生物群在人类新陈代谢中发挥作用.
脂肪组织和细菌存在之间的联系尚未完全理解.
已经检测到循环的细菌DNA,与血清细胞外囊泡 (EVs) 显示细胞癌 (RCC) 的诊断潜力.

研究的目的:

研究血清EVs中的细菌DNA与脂肪组织炎症之间的相关性.
确定血清EV中的细菌DNA配置文件是否可以作为RCC患者脂肪组织特征的生物标志物.

主要方法:

在77名RCC患者的血清EV中对细菌DNA的16SrRNA元基因组分析 (衍生队列).
关于EPC (Enterobacteriaceae,Polaromonas,Coxiellaceae) 索引的发展情况.
使用32名RCC患者的血清EVs验证EPC指数 (验证队列).

主要成果:

肠杆菌,波拉罗莫纳斯和可西拉属DNA的高表达与较低的梅奥粘着性概率 (MAP) 评分有关,这表明脂肪组织密度和粘附减少.
在导出 (AUC,0.76) 和验证 (AUC,0.81) 队列中,EPC指数有效地区分了低和高MAP得分组.
在预测低MAP分数方面,EPC指数表现出高灵敏度和特异性.

结论:

血清EV中的细菌DNA可以反映周围脂肪组织的炎症状态,特别是RCC患者的周围脂肪.
作为评估脂肪组织炎症和相关风险的非侵入性生物标志物,EPC指数显示出前景.

关键词:

脂肪组织的脂肪组织.细菌的 DNA 是细菌的 DNA.细胞外囊泡细胞外囊泡 rRNA基因 rRNA基因是一种基因.细胞癌瘤是细胞癌.

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