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Updated: Jun 7, 2025

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轻重稀土金属对抗氧化酶的调节:用催化酶进行的案例研究
Rashmi R Samal1, Umakanta Subudhi1
1Biochemistry & Biophysics Laboratory, Environment & Sustainability Department, CSIR-Institute of Minerals & Materials Technology, Bhubaneswar 751013, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
International journal of biological macromolecules
|November 20, 2024
概括
像 Praseodymium 化物和 Gadolinium 化物这样的稀土元素显著损害牛肝催化酶 (BLC) 的活性和稳定性. 这些类化合物与BLC结合,改变其结构并降低其分解过氧化的能力.
科学领域:
- 生物化学 生物化学
- 毒理学 毒理学 毒理学
- 频谱学是一种光谱学.
背景情况:
- 稀土元素 (REEs) 越来越多地用于各种应用,引发了人们对其环境和生物影响的担忧.
- 牛肝催化酶 (BLC) 是一种关键的酶,通过降解过氧化 (H2O2) 来参与细胞防御氧化应激.
研究的目的:
- 研究轻重稀土元素,特别是化 (PrCl3) 和化 (GdCl3) 对牛肝催化酶 (BLC) 的结构和功能的危险影响.
主要方法:
- 包括循环二元化 (CD) 和光谱在内的光谱技术被用来分析BLC的二级和三级结构的变化.
- 使用泽塔电位 (ζ) 测量,异热定位热量计 (ITC) 和分子对接研究来了解BLC-兰他尼德相互作用和结合部位.
- 酶活性测定和化温度 (Tm) 测定评估了功能和热稳定性的影响.
主要成果:
- PrCl3和GdCl3显著降低了BLC的催化效率,分别降低了约1.8和2.6倍.
- 光谱分析显示,α-螺旋的含量降低,随机线圈增加,表明结构性变质.
- 泽塔潜力逆转和ITC数据证实了自发的BLC-兰坦化复合体形成,对接研究表明酶的活性部位内结合.
- 在兰化物存在的情况下,BLC的化温度显著下降表明热稳定性降低.
结论:
- PrCl3和GdCl3对BLC产生危险影响,通过直接结合和变质,损害其酶活性和结构完整性.
- 这些发现表明,与REE暴露于生物系统相关的潜在风险存在,并突出了综合光谱和计算方法对评估这种对蛋白质的影响的有用性.
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