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Updated: Apr 30, 2026

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使用商业方法检测C. glabrata中的 Echinocandin 耐药性:CLSI 或 EUCAST 断点是否适合进行分类分类?

Pilar Escribano^1,2,3, Laura Alguacil Cuéllar^1,2, Almudena Álvarez-Gutiérrez^1,2

¹Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Universidad Complutense de Madrid, Madrid, Spain.

|November 20, 2024

概括

传感器的酵母One和Etest条纹有效地检测了Candida glabrata中的 echinocandin 耐药性. 这些方法具有很高的准确性,可以分类超过90%的分离物,这对于指导抗真菌治疗至关重要.

科学领域:

医学真菌学医学真菌学
抗微生物耐药性抗微生物耐药性
临床微生物学临床微生物学

背景情况:

在Candida glabrata中耐 Echinocandin 耐药性是一个重大的临床挑战.
之前对检测方法的研究受限于少量的耐药分离物.
准确的敏感性测试对于侵入性真菌感染的有效治疗至关重要.

研究的目的:

为了评估Sensititre YeastOne和Etest条带的性能,以检测Candida glabrata中的 echinocandin 耐药性.
通过基因型信息 (FKS测序) 和已确定的断点来评估这些方法的准确性.
将商业方法的性能与EUCAST和CLSI指南进行比较.

主要方法:

测试了80种Candida glabrata分离物,包括敏感和耐药菌株.
测序了FKS基因,以确认耐药机制.
使用Sensititre YeastOne和Etest条纹确定了 Echinocandin 的敏感性.
使用EUCAST和CLSI断点来解释最小抑制度 (MIC).

主要成果:

两种Sensititre YeastOne和Etest条带都表现出高准确度,正确分类超过90%的分离物.
在两种方法中,观察到对anidulafungin和micafungin耐药性的特定MIC范围.
分类中的错误很少发生,但在EUCAST (虚假抗性) 和CLSI (虚假易感性) 断点之间有所不同.

结论:

传感器酵母One和Etest条纹适用于检测Candida glabrata中的 echinocandin 耐药性.
包括大量的FKS突变体加强了研究的发现.
这些验证的方法可以帮助临床决策,并需要进一步的多中心验证.

关键词:

没有任何的idulafungin.甘虫 (Candida glabrata) 是一种有毒的植物.商业方法商业方法微粒菌在菌中的作用电阻的阻力可以

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