两个机制抑制了环素B1的翻译,以维持小鼠卵子细胞中的预相停止
Shiya Cheng1,2, Melina Schuh3,4
1Department of Meiosis, Max Planck Institute for Multidisciplinary Sciences, 37077, Göttingen, Germany.
Nature communications
|November 20, 2024
概括
哺乳动物卵细胞预酶停止依赖于休眠的环林B1信使RNAs (mRNAs) 的翻译控制. RNA结合蛋白DDX6,LSM14B,CPEB1和细胞质多A结合蛋白 (PABPCs) 维持了这一关键的生殖停止.
科学领域:
- 生殖生物学 生殖生物学
- 分子细胞生物学 分子细胞生物学
- 在RNA生物学,RNA生物学.
背景情况:
- 哺乳动物中的卵子细胞在 I 预相中停止,这是生长支持繁殖的关键阶段.
- 这种停止取决于抑制线粒激酶CDK1,但转化调节仍未得到研究.
研究的目的:
- 为了研究转化控制在哺乳动物卵细胞预发期I停止中的作用.
- 确定调节休眠环林B1信使RNA (mRNA) 翻译的机制.
主要方法:
- 截断技术用于识别与环林B1mRNA的蛋白质相互作用.
- 对RNA结合蛋白复合物的分析及其与环林B1mRNA的3'未翻译区域 (3'UTR) 的相互作用.
- 研究细胞质多A结合蛋白 (PABPCs) 在mRNA翻译调节中的作用.
主要成果:
- 预相停止是通过休眠环林B1mRNAs的翻译控制来调节的.
- 包括DDX6,LSM14B和CPEB1在内的复合物通过其3'UTR.直接抑制环林B1转化.
- 细胞质多A结合蛋白 (PABPCs) 通过隔离翻译机制间接抑制环素B1的翻译.
结论:
- RNA结合蛋白DDX6,LSM14B和CPEB1协调维持环林B1mRNA的休眠状态和卵细胞预酶的停止.
- 细胞质多A结合蛋白 (PABPCs) 在控制卵细胞停止期间的mRNA休眠过程中起着意想不到的作用.
- 这项研究揭示了对卵细胞成熟和生殖过程的转化调节的新见解.
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