一个改进的转录组注释揭示了非对称的表达和独特的调节模式在allotetraploid常见鱼
Qi Wang1, Meidi Huang Yang1, Shuangting Yu1
1Key Laboratory of Aquatic Genomics, Ministry of Agriculture and Rural Affairs, and Beijing Key Laboratory of Fishery Biotechnology, Chinese Academy of Fishery Sciences, Beijing, China.
Communications biology
|November 20, 2024
概括
在全类鱼中,B亚基因组显示出比A亚基因组更多的RNA编辑和替代拼接事件. 然而,lncRNAs和circRNAs在两个亚基因组中表现出类似的表达和功能,这表明它们有共同的调节机制.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 水产养殖是水产养殖的一种方式.
背景情况:
- 鱼有两个亚基因组 (A和B),之前已经观察到蛋白质编码基因的分歧表达.
- 在其他转录和转录后事件中亚基因组优势的程度在很大程度上仍未被探索.
研究的目的:
- 为了研究常见鱼在替代拼接,lncRNA,circRNA和RNA编辑事件中的亚基因组特异性差异.
- 使用先进的测序技术来完善普通鱼转录组注释.
主要方法:
- 使用Illumina RNA测序和PacBio全长测序来分析常见的鱼转录组.
- 在A和B亚基因组之间对四个关键的转录和转录后事件 (替代拼接,lncRNAs,circRNAs,RNA编辑) 的比较分析.
主要成果:
- B亚基因组表现出更高频率的替代拼接事件, lncRNAs 和 circRNAs.
- lncRNAs和circRNAs在子基因组之间的表达水平,组织特异性,序列特征或功能上没有显著差异.
- B亚基因组显示了更多的RNA编辑事件和更高的基基替代频率,在所有分析事件中观察到的功能偏差.
结论:
- 常见的鱼在其两个亚基因组之间表现出复杂和不平衡的表达策略.
- 虽然B亚基因组在替代拼接和RNA编辑方面更活跃,但这两种亚基因组对lncRNA和circRNA都有共同的调节机制.
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