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罗塔病毒重新连接宿主细胞的代谢途径,使其转向谷氨胺代谢,从而有效地感染病毒

Suvrotoa Mitra¹, Ratul Datta Chaudhuri¹, Rakesh Sarkar²

¹Division of Virology, ICMR-National Institute of Cholera and Enteric Diseases (presently ICMR-NIRBI), Kolkata, West Bengal, India.

|November 20, 2024

概括

罗塔病毒感染显著改变宿主细胞的新陈代谢,特别是对病毒复制至关重要的谷氨酸通路. 通过像氨基酸 (AOAA) 这样的化合物准谷氨胺代谢,显示出新抗病毒疗法的前景.

科学领域:

病毒学病毒学
细胞的新陈代谢
胃肠病学胃肠病学

背景情况:

罗塔病毒 (RV) 导致全球严重的腹死亡,疫苗的疗效因菌株多样性和宿主条件等因素而有所变化.
需要抗病毒策略来补充疫苗接种,并减少频繁的RV感染的负担.
了解宿主-病原体代谢相互作用对于开发向疗法至关重要.

研究的目的:

研究RV-SA11感染对人类肠道上皮细胞 (HT-29) 代谢的影响.
为了确定宿主代谢途径,对VR复制至关重要.
为了评估谷氨胺代谢作为潜在的治疗点对抗RV感染.

主要方法:

在RV-SA11感染后,探索HT-29细胞的内膜代谢面貌.
对宿主细胞代谢途径显著变化的分析.
在体外和体内评估RV-SA11复制使用氨基酸 (AOAA) 来抑制来自谷氨酸的阿斯巴酸生物发生.

主要成果:

RV-SA11感染显著改变了宿主代谢途径,包括核酸生物合成,氨酸/酸/酸代谢和酸循环.
发现RV复制完全依赖宿主谷氨胺代谢.
使用AOAA从谷氨酸中抑制阿斯巴酸生物发生,在体外和体内显著减少了RV-SA11感染.

结论:

RV感染深刻影响宿主细胞代谢,突出病毒对特定宿主途径的依赖.
主体谷氨胺代谢对VR复制至关重要,为治疗干预提供了可行的点.
氨基酸 (AOAA) 通过向宿主代谢途径,显示出作为抗病毒剂对抗轮状病毒的潜力.

关键词:

罗塔病毒 (Rotavirus) 是一种病毒.这是一种抗病毒药物.亚斯巴酸氨基转移酶谷氨胺的代谢过程代谢生物组的代谢生物组

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