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与小米相关的益生菌细菌的功能和分子特征
Bashipangu Gouthami1, Alaguthevar Ramalakshmi2, Murugesan Balakrishnan3
1Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, 641 003, India.
BMC microbiology
|November 20, 2024
概括
来自发酵米的原生乳酸细菌 (LAB) 被选为益生菌潜力. 五个分离物体显示出有希望的生存,疏水性和抗菌活性,其中三种被确定为潜在的益生菌:Weisella cibaria和Weisella confusa.
科学领域:
- 食品微生物学 食品微生物学
- 益生菌研究 益生菌研究
- 可持续的粮食生产可持续的粮食生产
背景情况:
- 乳酸细菌 (LAB) 对于可持续的食品生产至关重要,增强基质的生物可用性并产生对健康至关重要的益生菌.
- 来自发酵食品的本地LAB分离物为识别具有功能性质的新型益生菌菌株提供了宝贵的资源.
研究的目的:
- 选和描述从发酵中分离出的本地乳酸细菌 (LAB),以检测它们的益生菌潜力,安全性和抗菌活性.
- 确定具有重要的功能性质的LAB菌株,以作为食品和健康行业的益生菌潜在应用.
主要方法:
- 从发酵的小米中分离和净化33个LAB菌株.
- 基于殖民地形态,生化分析和催化酶活性进行表征.
- 在各种盐,pH和温度条件下对生长的评估.
- 评估益生菌特性,包括耐酸性和胆盐耐受性,以及表面疏水性.
- 对病原性真菌 (Aspergillus flavus) 和细菌进行抗菌活性测试,并进行抗生素耐药性分析.
主要成果:
- 鉴定出13个阴性催化酶的LAB分离物,其中5个在测试条件下表现出最佳生长 (6.5-9.5%盐,pH4.5-8.5,17-40°C).
- 这五种分离物在酸 (56.2-73.7%) 和胆盐 (55.3-70.3%) 度下表现出良好的生存率,表面疏水性在41-75%之间.
- 四个分离物显示出对阿斯伯吉卢斯黄菌有显著的抗真菌活性. 三个分离物体表现出强烈的抗菌活性,并产生超过参考菌株的抗菌化合物. 抗生素耐药性各不相同,抗阿莫西林,克洛克萨西林和青素-V,但大多数人对青素和四环素敏感.
结论:
- 五种来自发酵的本地LAB分离物具有显著的益生菌特征,包括对胃肠道疾病的耐受性和抗菌性质.
- 鉴定出Weisella cibaria (ME9,ME26) 和Weisella confusa (ME25) 是益生菌应用的有前途的候选物种.
- 这些发现强调了小米相关的LAB作为开发功能性食品和促进健康的益生菌的可持续来源的潜力.
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