系统和转录反应间歇性禁食和模仿禁食的饮食在小鼠
Helene Michenthaler1, Kalina Duszka2, Isabel Reinisch3
1Division of Cell Biology, Histology and Embryology, Gottfried Schatz Research Centre, Medical University of Graz, Graz, Austria.
BMC biology
|November 20, 2024
概括
使用间歇性禁食 (IF) 或模仿禁食饮食 (FMD) 的饮食限制 (DR) 显示了没有不良影响的好处. 两者都激活了类似的肝脏通路,但口病会诱导更强的初始反应,而重复的口病会抑制一些基因表达.
科学领域:
- 对饮食干预的代谢调节和转录反应.
- 哺乳动物模型中禁食方案的比较分析.
背景情况:
- 饮食限制 (DR) 为健康和长寿带来好处,并提高治疗效果.
- 存在多种不同的DR饮食,需要标准化响应测量.
- 研究小鼠对IF和口炎的系统和肝脏转录反应.
研究的目的:
- 为了比较间歇性禁食 (IF) 和仿禁食饮食 (FMD) 的全身和肝脏特异性转录反应.
- 在小鼠中评估这些饮食限制方法的短期和长期影响.
主要方法:
- 使用C57BL/6J小鼠进行短期和长期研究.
- 进行间歇性禁食 (IF) 和商用禁食模仿饮食 (FMD).
- 采用RNA测序来分析肝脏特异性的转录反应和测量血液参数.
主要成果:
- 无论是IF还是口病都没有造成不良影响;体重减轻是暂时的,并在重新养期间得到补偿.
- 与IF相比,口疾病诱导了更大的体重减轻和更强的禁食反应 (较低的葡萄糖,较高的类素,FFAs,FGF21).
- 这两种疗法都激活了类似的肝脏通路,特别是PPARα信号传递. 关节炎更强烈地提高了葡萄糖生成,PPARα和p53向基因的调节. 重复的口病周期使基因生成和脂肪酸氧化基因表达变得模糊,与重复的IF不同.
结论:
- 短期口病产生更明显的系统性变化和略高于IF的体重减轻,两者都激活了类似的肝脏关键通路,如PPARα信号传递.
- 胃口炎引起更强的个体基因转录反应,但周期性胃口炎的应用特别阻碍了脂肪酸氧化和生成基因的上调.
- 无论是IF还是口病都是有效的DR疗法,为禁食研究提供了有价值的比较数据.
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