一个双诱导的控制系统,用于多步生物合成途径
Andrés Felipe Carrillo Rincón1,2, Alexandra J Cabral1, Andras Gyorgy2
1Department of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, MA, USA.
Journal of biological engineering
|November 20, 2024
概括
这项研究开发了增强的遗传工具,即sigma70 (σ70) lac和tet表达系统,用于微生物细胞工厂. 这些系统与脚开关相结合,改善了对天然产品生物合成的基因表达的控制.
科学领域:
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
- 微生物生物技术 微生物生物技术
背景情况:
- 工业天然产品合成需要强大的微生物底盘和有效的遗传工具.
- 多功能和便携式遗传工具简化了已建立的微生物中的化合物生产.
- 西格玛70 (σ70) 乳酸和酸表达系统在格拉姆阴性细菌中提供受调节的重组蛋白质表达.
研究的目的:
- 开发和评估便携式遗传工具,用于控制多基因生物合成途径.
- 增强sigma70 (σ70) 的lac和tet表达系统,以改善重组蛋白质的生产.
- 评估这些系统在各种微生物底盘中的组合控制和代谢影响.
主要方法:
- 在单个等离子体上结合sigma70 (σ70) lac和tet表达系统.
- 使用光报道器,烯和β-胡卜素生产来评估性能.
- 集成的脚开关用于翻译控制和增强的基因表达调节.
- 使用大肠杆菌,伪杆菌和自然杆菌作为微生物底盘.
主要成果:
- 双西格玛70 (σ70) 的lac和tet表达系统展示了组合控制能力.
- 手持开关显著改善了动态范围和基底转录控制.
- 改进的系统使生物合成中间体烯的积累成为可能.
- 代谢成本和协同效应在不同的微生物宿主中具有特征.
结论:
- 开发了多功能,便携式遗传工具,用于控制马蛋白细菌中的多基因通路.
- 增强的sigma70 (σ70) 表达系统与脚开关有助于酶和天然产品的生物合成.
- 这些工具为微生物细胞工厂应用和自然产品发现提供了宝贵的资源.
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