铁氧化物纳米颗粒激活树突细胞取决于细胞外环境
Mason Song1, Robert Ivkov2,3,4,5, Preethi Korangath2,3
1Department of Biomedical Engineering, Whiting School of Engineering, Johns Hopkins University Baltimore 21218 USA.
Nanoscale advances
|November 21, 2024
概括
基乙烯粉涂层的氧化铁纳米颗粒在体外没有激活树突细胞 (DCs). 在体内,只有脏和骨髓DCs表现出激活,突出显示了对纳米粒子的特定环境免疫反应.
科学领域:
- 免疫学 免疫学 免疫学
- 纳米医学是一种纳米医学.
- 材料科学 材料科学 材料科学
背景情况:
- 纳米粒子可以调节宿主免疫反应,而细胞的吸收是关键事件.
- 纳米粒子诱导免疫反应的基础机制,特别是树突细胞 (DC) 激活,需要进一步阐明.
- 以前的研究表明,系统性纳米粒子暴露可以引起抗瘤适应性免疫力.
研究的目的:
- 在体外和体内研究纳米粒子介导的树突细胞 (DC) 激活.
- 评估基乙烯粉涂层氧化铁纳米颗粒对DC成熟和功能的影响.
- 在小鼠模型中评估这些纳米颗粒的毒性.
主要方法:
- 在FVB/NJ小鼠中对粉涂层的Nanoferrite®生物化纳米颗粒 (BP) 的毒性评估.
- 在与BP进行化后对DC成熟标记物 (CD86,MHC II) 的体外评估.
- 在活体中对注射BP的小鼠的各种器官中的DC激活标记物 (CD80,CD86,MHC II) 的分析.
主要成果:
- 在每只小鼠服用高达25毫克Fe的剂量时,没有观察到显著的毒性.
- 在体外,无论是JAWSII DCs还是骨髓衍生DCs (BMDCs) 在BP暴露时都没有表现出成熟,尽管纳米粒子吸收率很高.
- 在体内,只有脏和骨髓DCs在BP注射后表现出激活标记的增加;来自其他器官的DCs没有.
结论:
- 基乙烯粉涂层的氧化铁纳米颗粒在体外没有诱导显著的DC成熟.
- 在体内纳米粒子暴露导致特定环境的DC激活,主要在脏和骨髓中.
- 在体外发现可能无法准确预测体内对纳米颗粒的免疫反应,这强调了体内验证的必要性.
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