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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Updated: Jun 7, 2025

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

样本复杂化用于视网膜单细胞RNA测序.

Justin Ma¹, Ting-Kuan Chu², Maria Polo-Prieto³

¹Department of Pathology and Immunology, Baylor College of Medicine, Houston, TX, USA.

|November 21, 2024

概括

本研究引入了一种样本多重复合管道,用于小鼠视网膜质细胞的单细胞RNA测序 (scRNA-seq). 这种方法有效地标记样品,使细胞类型分布和变异的精确分析,而不会丢失来源信息.

关键词:

分子生物学分子生物学神经科学是一个神经科学.俄米克斯 (Omics) 是一个电子游戏.文字转录学 (Transcriptomics) 是一个学科.

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Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Published on: May 22, 2017

相关实验视频

Last Updated: Jun 7, 2025

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Published on: May 22, 2017

科学领域:

基因组学就是基因组学.
神经科学是一个神经科学.
生物信息学是一种生物信息学.

背景情况:

通过单细胞RNA测序 (scRNA-seq) 来表征罕见细胞群是具有挑战性的.
目前的方法通常需要样本丰富和聚合,失去关键样本来源和表型数据.
这在疾病模型或人类样本研究等高变异实验中尤其具有问题.

研究的目的:

开发和验证一个scRNA-seq样本复合管道用于小鼠视网膜质细胞.
为了能够精确分析细胞类型分布和跨多个样本的转录组差异.
为了证明多重复合用于识别scRNA-seq数据中的多重体的实用性.

主要方法:

建立了一个样本多重复合管道,使用胆固醇修饰的小分子用于scRNA-seq.
将管道应用于小鼠视网膜质细胞.
生物信息学解决的独特序列条形码来识别样品来源.

主要成果:

从多重样本的scRNA-seq数据中获得了更高的精度.
在视网膜样本中研究了细胞类型分布和转录组变异.
证明了管道在识别非标记样本中的多重体方面的有效性.

结论:

用胆固醇修饰的寡合物进行样本复合是scRNA-seq.q.的可行策略.
这种方法克服了丰富和聚合的局限性,保留了样本来源和表型数据.
开发的管道增强了对罕见细胞群和复杂实验设计的scRNA-seq分析.