在Periplaneta americana细胞中对复合蛋白表达的分子机制的基于转录组的分析
Chenjing Ma1, Xin Zhang1, Xian Li1
1Key Laboratory of Breeding and Utilization of Resource Insects of National Forestry and Grassland Administration, Institute of Highland Forest Science, Chinese Academy of Forestry, Kunming, China.
The FEBS journal
|November 21, 2024
概括
昆虫细胞系RIRI-PA1比Sf21细胞更快地产生更高的重组蛋白产量. 这种增强的产生与早期感染Autographa californica多重核聚合体病毒 (AcMNPV) 期间更大的核糖体路径基因表达有关.
科学领域:
- 生物技术是生物技术.
- 分子生物学分子生物学
- 昆虫细胞培养 昆虫细胞培养
背景情况:
- 昆虫细胞-细菌病毒表达载体系统 (IC-BEVS) 是生产蛋白质,疫苗和基因治疗载体的关键工具.
- IC-BEVS的局限性包括狭窄的宿主范围和低于最佳的蛋白质产量.
- 以前已经证明,来自Periplaneta americana的RIRI-PA1细胞系产生了更高的重组蛋白质生产.
研究的目的:
- 与Sf21细胞相比,阐明RIRI-PA1细胞中更高的重组蛋白产量背后的分子机制.
- 识别参与重组蛋白表达的差异表达基因 (DEG) 和调节途径.
- 探索提高IC-BEVS效率的潜在应用.
主要方法:
- 感染AcMNPV-GFP的RIRI-PA1和Sf21细胞的RNA测序和转录组分析.
- 在感染后24,72和168小时对基因表达的分析.
- 基因本体学 (GO),eggNOG和DEG的KEGG注释,以确定监管候选人.
主要成果:
- 在感染后的RIRI-PA1和Sf21细胞之间观察到DEGs的显著差异.
- 感染后24小时,RIRI-PA1细胞显示与核糖体蛋白相关的DEGs (7:12比) 的丰富度较高,与较高的蛋白质生产相关.
- 基因表达模式与重组蛋白质合成相关,涉及核糖体,Toll和Imd信号传递,以及细胞染色体P450通路.
结论:
- 核糖体通路在早期RIRI-PA1细胞感染AcMNPV.在调节蛋白质表达过程中起着至关重要的作用.
- 这些发现表明,有可能对工程昆虫细胞系进行改造,以优化重组蛋白质生产时间表.
- 对RIRI-PA1细胞反应的更好理解可以促进IC-BEVS的应用和效率.
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