来自Streptomyces thermodiastaticus菌株的热稳定细胞酶的优化生产和表征
Mery S Waheeb1, Walid F Elkhatib2,3, Mahmoud A Yassien2
1Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Organization of African Unity St., POB: 11566, Abbassia, Cairo, Egypt. merysameh@yahoo.com.
AMB Express
|November 21, 2024
概括
这项研究利用统计方法和马辐射优化了Streptomyces thermodiastaticus的细胞酶生产,使酶产量显著增加了5.1倍. 增强的细胞酶具有较高的耐热性和特异性活性.
科学领域:
- 微生物学 微生物学
- 酶技术 酶技术是一种
- 生物技术是生物技术.
背景情况:
- 细胞酶对于生物质降解至关重要.
- 优化微生物细胞酶的生产对于工业应用至关重要.
- 热杆菌 (Streptomyces thermodiastaticus) 是一种高性能纤维酶的潜在来源.
研究的目的:
- 识别和优化影响Streptomyces thermodiastaticus细胞酶生产的因素.
- 通过统计优化和玛辐射突变性来提高细胞酶产量.
- 为了描述纯化的细胞酶的特性.
主要方法:
- 使用16S rRNA基因测序,分离和识别细胞酶产生细菌.
- 一个因素一次 (OFAT) 和响应表面方法 (RSM) 用于媒体优化.
- 玛辐射用于菌株突变和酶生产增强.
- 硫酸沉,透析,SDS-PAGE,以及用于酶净化和表征的生殖图.
主要成果:
- Streptomyces thermodiastaticus TS4 被确定为高细胞酶生成的分离物.
- 在优化条件下 (pH 6.0,2% CMC,0.03% ,0.12% NaCl) 细胞酶的生产率提高了 3.24 倍 (2023 U/L).
- 玛辐射 (4KGy) 与优化相结合,使细胞酶产量增加了5.1倍.
- 部分净化的纤维素 (63 kDa) 在60°C和pH值为5.0时表现出最佳活性,具有高达90°C的显著耐热性.
结论:
- 统计优化和玛辐射是增强Streptomyces thermodiastaticus细胞酶生产的有效策略.
- 描述的细胞质具有工业应用的理想性质,包括高热耐受性.
- 这项研究为大规模生产和利用微生物细胞酶提供了基础.
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