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Updated: Jun 12, 2025

Western Blotting: Sample Preparation to Detection
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Western Blotting: Sample Preparation to Detection

Published on: October 14, 2010

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在非压力和压力条件下检测和定量hATF4-HA的协议,使用自动化和定量化的Jess西部抹杀

Anna M Smirnova1, Anna Herrmannová1, Leoš Shivaya Valášek1

  • 1Laboratory of Regulation of Gene Expression, Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, Prague 142 20, Czech Republic.

STAR protocols
|November 21, 2024
PubMed
概括

这项研究引入了一种用于测量细胞应激期间激活转录因子4 (ATF4) 水平的新方法. 该协议使ATF4的精确量化成为可能,克服了传统参考基因的挑战.

关键词:
细胞培养培养的细胞培养.基因表达的基因表达方式蛋白质表达和净化过程

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Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
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