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一个离网格的离散模型来描述细丝酵母菌群的形态

Kai Li¹, J Edward F Green¹, Hayden Tronnolone²

¹School of Computer and Mathematical Sciences, University of Adelaide, Adelaide SA, Australia.

PLoS computational biology

|November 21, 2024

概括

酵母细胞通过切换芽模式在压力下形成纤维. 数学建模和实验揭示了殖民地大小和细胞分叉驱动这种丝状生长,与观察到的模式密切匹配.

科学领域:

微生物学和数学生物学
研究细胞机制和殖民地发展.

背景情况:

麦芽菌体表现出表型可塑性,在压力下从双极芽转变为单极假象芽生长.
这种过渡导致长长的细胞形成细丝,使殖民地扩张和营养食成为可能.
了解这种形态变化的驱动因素对于细胞生物学和发育研究至关重要.

研究的目的:

量化地将单个细胞芽机制与酵母菌群中的新兴空间模式联系起来.
推断管理转变为伪头生长和扩散的关键参数.
通过对殖民地形态学的实验观测来验证数学模型.

主要方法:

开发一种基于非格子代理的酵母殖民地生长的数学模型.
使用实验数据对参数推理应用近似贝叶斯计算.
在不同的营养条件下,分析了两种S. cerevisiae菌株的酵母殖民地图像.

主要成果:

鉴定了芽过渡时期的殖民地大小和伪分叉作为形态学的关键因素.
推断模型参数准确地复制了实验殖民地形状.
证明了计算模型对酵母殖民地发展的预测能力.

结论:

这项研究成功地整合了数学建模和实验,以阐明酵母线状生长.
控制殖民地形态的关键细胞机制已被确定和量化.
这些发现为了解和预测环境影响下的微生物殖民地发展提供了一个框架.

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