相关实验视频

Updated: Jun 7, 2025

Experimental Melanoma Immunotherapy Model Using Tumor Vaccination with a Hematopoietic Cytokine

Experimental Melanoma Immunotherapy Model Using Tumor Vaccination with a Hematopoietic Cytokine

Published on: February 24, 2023

血小板因子4诱导的T_H1-T_reg两极化抑制抗瘤免疫力

Ayumi Kuratani^1,2, Masaaki Okamoto^1,2, Kazuki Kishida^3,4

¹Department of Immunoparasitology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan.

Science (New York, N.Y.)

|November 21, 2024

概括

通过分泌血小板因子4 (PF4) 来促进表达酶I的瘤相关巨细胞. 这种化学基因驱动T辅助1极化调节性T细胞 (TH1-Treg细胞) 的积累,阻碍抗瘤免疫力.

科学领域:

免疫学
癌症生物学
瘤微环境研究

背景情况:

瘤微环境 (TME) 通常含有免疫抑制细胞,包括T辅助1极化调节T细胞 (TH1-Treg细胞).
在TME中驱动TH1-Treg细胞的丰富机制仍然不完全理解.

研究的目的:

阐明TH1-Treg细胞在TME中积累的机制.
在调节TH1-Treg细胞群和瘤生长中研究表达酶I的瘤相关巨细胞 (Arg1+ TAMs).

主要方法:

在瘤模型中选择性耗尽Arg1+TAM.
在TME中分析TH1-Treg细胞比率.
通过Arg1+TAM检测血小板素分泌 (血小板素因子4,PF4) 的情况.
通过CXCR3和IFN-γ受体信号传递对TH1-Treg细胞极化中的PF4作用的评估.
基因失活和中和PF4.

主要成果:

减少Arg1+TAMs抑制了瘤生长,并减少了TH1-Treg细胞在TME中的比例.
发现Arg1+ TAMs分泌PF4,一种增强干扰素-γ (IFN-γ) 诱导的Treg细胞偏离到TH1-Treg细胞的化学激素.
这种偏向取决于CXCR3和IFN-γ受体.
基因PF4失活或中和显著降低TH1-Treg细胞的积累和瘤的生长.

结论:

由Arg1+TAM衍生的PF4是TME中TH1-Treg细胞积累的关键驱动因素.
这种机制有助于抑制免疫力,促进瘤生长.
向PF4介导途径可能是增强抗瘤免疫力的新疗法.

更多相关视频

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Published on: November 28, 2019

Functional Characterization of Regulatory Macrophages That Inhibit Graft-reactive Immunity

Functional Characterization of Regulatory Macrophages That Inhibit Graft-reactive Immunity

Published on: June 7, 2017

相关概念视频

Tumor Immunotherapy

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.

T Cell Types and Functions

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...