外体LncRNA和CircRNA通过竞争性内源性RNA机制调节外周血液单核细胞功能,用于过敏性鼻炎
Guangyao Mao1, Qian Zhu2, Yiyun Zeng2
1Department of Central Laboratory, The Affiliated Taizhou People's Hospital of Nanjing Medical University, Taizhou, China, 1563817606@qq.com.
International archives of allergy and immunology
|November 21, 2024
概括
功能障碍的外周血液单核细胞 (PBMCs) 导致过敏性鼻炎 (AR). 这项研究在外体中确定了一种关键的长非编码RNA (lncRNA),可以调节AR患者的PBMC功能.
科学领域:
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
- 基因组学就是基因组学.
背景情况:
- 周围血液单核细胞 (PBMCs) 功能障碍与过敏性鼻炎 (AR) 病原发生有关.
- 来自血的外体含有调节性RNA,可能会影响AR中的PBMC功能.
研究的目的:
- 研究PBMC中的竞争性内源RNA (ceRNA) 网络,其中包括来自AR相关等离子体外体的长非编码RNA (lncRNA) 和圆形RNA (circRNA).
- 确定潜在的诊断生物标志物和AR的治疗点.
主要方法:
- 高通量测序PBMC和等离子体外基因组,以识别差异表达RNA.
- 生物信息分析用于构建ceRNA网络.
- 定量逆转录聚合酶连锁反应 (qRT-PCR) 和受体运行特征 (ROC) 曲线分析用于验证和诊断评估.
- 在体外测定使用培养PBMC和双露西法酶记者测定.
主要成果:
- 构建了6个ceRNA网络,影响了PBMC化学排斥活性和JAK-STAT信号.
- 一种特定的lncRNA,ENST00000650850,在AR患者的血外体中下调,显示出诊断潜力.
- ENST00000650850表达与PBMC中的IL6mRNA正相关,AR外体在健康的PBMC中减少了IL6RmRNA.
结论:
- 血外体中的lncRNA ENST00000650850可以通过miR-6747-3p调节PBMC中的IL6RmRNA.
- 这种调节机制可能会影响PBMC的功能,并有助于AR的发病和进展.
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