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在Penaeus vannamei中,WSSV诱导Rubicon表达以调节先天免疫反应

Phattarunda Jaree¹, Thaneeya Nantapojd¹, Chalermporn Ongvarrasopone¹

¹Center of Applied Shrimp Research and Innovation, Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom, 73170, Thailand.

Fish & shellfish immunology

|November 21, 2024

概括

中的Rubicon蛋白 (PvRUBCN) 对抗白斑综合征病毒 (WSSV) 的抗病毒免疫非常重要. 抑制PvRUBCN可以通过降低病毒载量和调节免疫反应来提高的生存率.

科学领域:

水生动物的健康状况.
传染性疾病传染性疾病
分子免疫学分子免疫学

背景情况:

鲁比孔蛋白质参与病毒复制.
白斑综合征病毒 (WSSV) 在养殖中造成重大损失.
鲁比康在对WSSV免疫力的作用尚不清楚.

研究的目的:

为了调查Penaeus vannamei Rubicon (PvRUBCN) 在WSSV感染期间的作用.
确定PvRUBCN在调节的亡和天生的免疫力中的功能.

主要方法:

克隆和对PvRUBCN基因进行测序.
在WSSV挑战下分析PvRUBCN表达模式.
使用dsRNA抑制PvRUBCN表达并评估WSSV负载和的存活率.
评估对亡相关 (caspase3) 和抗病毒 (Vago4,Vago5) 基因表达的影响.

主要成果:

确定了PvRUBCN基因,其蛋白质含有保存的域.
PvRUBCN表达是由WSSV感染引起的,主要局部在神经组织中.
抑制PvRUBCN显著降低了WSSV拷贝数量,增加了的存活率,降低了caspase3和干扰素类基因 (Vago4,Vago5).
氧化酶通路基因 (PPO1,PPO2) 没有受到影响.

结论:

PvRUBCN在对WSSV的抗病毒免疫力中起着至关重要的作用.
PvRUBCN对于WSSV的复制和病原发生是必不可少的.
准PvRUBCN可能是鱼中WSSV疾病管理的潜在策略.

关键词:

抗病毒免疫抗病毒免疫细胞灭亡 (apoptosis) 是一种死亡的过程.干扰素类似基因的基因.太平洋白是什么意思白色斑点综合征病毒病毒.dsRNARNA是什么意思

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