聚乙烯微塑料和聚烯纳米塑料的老化对抗生素耐药性基因转移在初级污泥发酵过程中的影响
Romana Saila1, Bipro Ranjan Dhar1
1Civil and Environmental Engineering, University of Alberta, 116 Street NW, Edmonton, AB T6G 1H9, Canada.
The Science of the total environment
|November 21, 2024
概括
在污泥发酵过程中,老化的纳米和微塑料 (NP / MP) 显著提高了挥发性脂肪酸的产生和抗生素耐药性的基因. 陈旧的聚乙烯微塑料对微生物群落产生负面影响,突出显示了陈旧塑料污染的风险.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 环境化学环境化学
背景情况:
- 纳米和微塑料 (NP/MP) 正在堆积在废水处理厂的污泥中.
- 陈旧的NP/MP对污泥发酵的影响尚不清楚.
研究的目的:
- 研究原始和老化的聚乙烯微塑料 (PEMPs) 和聚烯纳米塑料 (PsNPs) 对初级污泥发酵的影响.
- 评估老年NP/MP对挥发性脂肪酸 (VFA) 生产,抗生素耐药性基因 (ARG) 和微生物社区结构的影响.
主要方法:
- 在受控条件下 (pH 10,15天) 使用原始和经过气候变化的PEMPs和PsNPs进行了批量发酵实验.
- 气候变化涉及两周暴露在和酸中.
- 分析了VFA产量,ARG水平 (准tetA和tetM),以及微生物群落结构.
主要成果:
- 与原始样本相比,老化的PEMPs/PsNPs显著增加了VFA产量,老化的混合物上升了23.12%.
- 在所有NP/MP治疗中,ARG的相对丰度和总度都增加了,而老年PEMP的增长最为显著.
- 陈旧的PEMP对微生物群落产生了不利影响,使其丰富性和多样性下降,而原始的NP/MP和陈旧的PsNP则表现出不同的影响.
结论:
- 陈旧的纳米/微塑料对污泥发酵的影响比原始的纳米/微塑料更大.
- 该研究强调了老化塑料形式,微生物动态和污泥中ARG的扩散之间的复杂相互作用.
- 浮游生物,蛋白质细菌和TM7被确定为ARG的潜在宿主.
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