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相关概念视频

Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

10.6K
Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...
10.6K
Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

9.8K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
9.8K
Fixation and Sectioning01:03

Fixation and Sectioning

4.2K
Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
4.2K
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

4.0K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
4.0K
Simple Staining Technique01:24

Simple Staining Technique

2
OverviewStaining techniques in microscopy enhance the visualization of microorganisms by increasing contrast and allowing the differentiation of cellular structures. Simple staining is one of the fundamental methods used to observe the basic morphological characteristics of microorganisms, including their size, shape, and arrangement. This method relies on the application of a single dye to stain the entire cell, producing a clear contrast between the cell and the background.FixationFixation is...
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相关实验视频

Updated: Jun 6, 2025

Histological-Based Stainings Using Free-Floating Tissue Sections
06:45

Histological-Based Stainings Using Free-Floating Tissue Sections

Published on: August 25, 2020

18.4K

免疫染的简要指南 免疫染

Gyutae Park1, Sieun S Kim1, Jiwon Shim2

  • 1Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, South Korea.

Molecules and cells
|November 21, 2024
PubMed
概括
此摘要是机器生成的。

本指南解释了免疫染色,这是一种关键的生物技术,用于使用抗体可视化细胞和组织中的抗原定位和水平. 它涵盖了研究人员的原则,方法,类型和优化策略.

关键词:
免疫细胞化学 免疫细胞化学免疫光效应 免疫光效应免疫组织化学 免疫组织化学

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Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons
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Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons

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Immunohistochemistry on Paraffin Sections of Mouse Epidermis Using Fluorescent Antibodies
11:27

Immunohistochemistry on Paraffin Sections of Mouse Epidermis Using Fluorescent Antibodies

Published on: January 8, 2008

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相关实验视频

Last Updated: Jun 6, 2025

Histological-Based Stainings Using Free-Floating Tissue Sections
06:45

Histological-Based Stainings Using Free-Floating Tissue Sections

Published on: August 25, 2020

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Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons
07:30

Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons

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Immunohistochemistry on Paraffin Sections of Mouse Epidermis Using Fluorescent Antibodies
11:27

Immunohistochemistry on Paraffin Sections of Mouse Epidermis Using Fluorescent Antibodies

Published on: January 8, 2008

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科学领域:

  • 生物技术是生物技术.
  • 细胞生物学 细胞生物学
  • 免疫学 免疫学 免疫学

背景情况:

  • 免疫覆盖是生物研究中的一个基本技术.
  • 它可视化细胞或组织内特定分子 (抗原) 的位置和数量.
  • 抗体是检测抗原的关键试剂.

研究的目的:

  • 为免疫染色技术提供简要指南.
  • 解释底层原则和各种方法.
  • 提供关于常见挑战和优化的实用建议.

主要方法:

  • 该手稿概述了标准的免疫染色协议.
  • 它详细介绍了抗原检索和抗体化方法.
  • 讨论了不同的检测系统和可视化策略.

主要成果:

  • 该指南涵盖了直接和间接的免疫染方法.
  • 它解决了固定,透和阻塞的步骤.
  • 包括常见的陷和故障排除提示.

结论:

  • 此资源有助于研究人员理解和执行免疫染色.
  • 它增强了生物研究中的实验设计和数据解释.
  • 有效的免疫染色对于准确的分子定位和量化至关重要.