相关实验视频
Updated: Jun 6, 2025

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Physiology Lab Demonstration: Glomerular Filtration Rate in a Rat
Published on: July 26, 2015
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使用d-serine和d-asparagine测定尿液体积和淋巴膜过率
Ryo Tanaka1, Shinsuke Sakai2, Ayumu Taniguchi3
1Department of Urology, Osaka University Graduate School of Medicine, Suita, Japan.
概括
淋巴膜过率 (GFR) 可以准确地测量使用d-serine和d-asparagine来估计尿液体积. 这种方法提高了GFR测量的准确性,克服了传统尿液收集的挑战.
科学领域:
- 腎臟病學 (nephrology) 是一種醫學專業.
- 生物化学 生化学
- 分析化学 分析化学
背景情况:
- 淋巴膜过率 (GFR) 的测量通常受到不准确的尿液收集的限制.
- 由于最小的管状分泌/再吸收,D-氨酸和d-氨酸的清除被提议作为准确的GFR测量.
研究的目的:
- 假设d-serine和d-asparagine可以准确地确定尿液体积和GFR.
- 用d-serine和d-asparagine水平建立估计尿量 (eUV) 的方程.
- 用估计的尿量重新评估GFR测量.
主要方法:
- 对209名移植捐赠者/接受者进行横截面观察性研究.
- 通过胰岛素清除量测量的GFR.
- 开发了一个基于尿液d-serine和d-asparagine度的eUV方程.
- 用eUV取代异常尿量,用于GFR重新评估.
主要成果:
- 与肌素相比,D-氨酸和d-氨酸的清除显示了较小的比例偏差.
- 已确定的eUV方程是:21.88 /尿液d-Ser (0.40 + 0.20 × log10 ((尿液d-Asn)).
- 确定了20例明显的尿量变化;纠正的GFR (调整的mGFR) 在最初测量的GFR (mGFR) ~20mL/min/1.73m2内.
结论:
- D-氨酸和d-阿斯巴拉金几乎完全被排泄,从而能够准确地估计尿液体积.
- 这些氨基酸可用于估计尿量和正确GFR测量.
- 通过使用清除技术,eUV方法提高了GFR测定的准确性.
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