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通过向miR-18a-5p/AFF4轴,FENDRR抑制了膀癌细胞的扩散,干细胞,迁移,入侵和EMT过程

Changyuan Dai¹, Qingwen Li², Lili Wang³

¹Department of Urology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, 233000, Anhui, China.

Biochemical genetics

|November 21, 2024

概括

长非编码RNAFENDRR通过通过miR-18a-5p/AFF4通路抑制细胞增殖,迁移,入侵,干细胞和EMT来抑制膀癌的进展.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

膀癌 (BC) 是一种普遍存在的尿路恶性瘤,其复发率和死亡率很高.
长非编码RNAs (lncRNAs) 显示出作为癌症治疗的新生物标志物的潜力.
了解BC中lncRNAs的调节作用对于开发新的治疗策略至关重要.

研究的目的:

研究IncRNA胎儿致死非编码发育调节RNA (FENDRR) 在膀癌中的功能.
阐明FENDRR在调节BC细胞表型中的潜在分子机制.
探索FENDRR作为膀癌治疗点的潜力.

主要方法:

定量实时PCR (RT-qPCR) 用于基因表达分析.
亚细胞分离和光在现场杂交用于FENDRR局部化.
细胞分析包括EDU,球体形成,Transwell入侵和伤口愈合.
对于表皮细胞-介质细胞过渡 (EMT) 标记物的西部涂抹.
路西法雷斯记者测量以确认分子相互作用.

主要成果:

在BC组织和细胞系中,FENDRR表达显著下调.
主要在BC细胞的细胞质中定位的FENDRR.
过度表达FENDRR抑制了BC细胞的增殖,迁移,入侵,干细胞和EMT.
基金直接针对miR-18a-5p,这反过来又针对AFF4.
抑制AFF4部分扭转了FENDRR.的抑制作用.

结论:

在膀癌中,FENDRR作为瘤抑制剂起作用.
通过调节miR-18a-5p/AFF4轴,FENDRR可以抑制BC的进展.
芬德尔代表了膀癌治疗的潜在治疗标.

关键词:

AFF4 AFF4 AFF4 AFF4 AFF4 AFF4 AFF4 AFF4 AFF4 AFF4 AFF4 AFF4 AFF4 AFF4 AFF4 膀癌是一种癌症.在EMT中,EMT是EMT.在FENDRRR中,我们可以看到.在米R-18a-5p.

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