扩大细菌的基因组信息,用于生物合成基因集群发现
Lijie Song1, Lasse Johan Dyrbye Nielsen1, Xinming Xu1,2
1DTU Bioengineering, Technical University of Denmark, 2800 Kgs, Lyngby, Denmark.
Scientific data
|November 21, 2024
概括
这项研究介绍了来自子形成细菌Bacillales的121个新基因组,揭示了许多生物合成基因集群. 这些数据扩大了对农业和医学微生物天然产品发现的理解.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 生物技术是生物技术.
背景情况:
- 培育子的Bacillales细菌是各种二次代谢物的丰富来源,在农业,生物技术和医学中具有应用.
- 二次代谢产物由生物合成基因集群 (smBGCs) 编码,它们对新生物活性化合物发现具有重大研究兴趣.
研究的目的:
- 来自全球来源的子形成菌株Bacillales的121个新组装的基因组.
- 识别和描述这些基因组内的二次代谢物生物合成基因集群.
- 为进一步研究Bacillales的代谢潜力提供一个开放的数据集.
主要方法:
- 基因组组装使用牛津纳米孔长读和MGI短读测序技术.
- 生物分类学分析以确定数据集中所代表的属和物种.
- 在基因组采矿中,使用生物信息学工具预测和识别生物合成基因集群 (BGCs).
主要成果:
- 组装了121个新基因组,包括62个完整的基因组,2个染色体层次的基因组和57个结合层次的基因组.
- 这些基因组代表了16个属,其中大多数 (69) 属于Bacillus属.
- 总共有1176个预测的BGC在数据集中被确定,突出显示了新代谢物发现的潜力.
结论:
- 提出的数据集显著扩大了可用于子形成的细菌体的基因组信息.
- 已识别的BGC为探索新的生物活性化合物提供了宝贵的资源.
- 这些开放访问的数据将有助于更深入地了解细菌中二次代谢物生产的遗传基础.
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