候选血清蛋白生物标志物用于在结核流行环境中诊断活性肺结核
Sosina Ayalew1,2, Teklu Wegayehu3, Biniam Wondale3
1Armauer Hansen Research Institute, Addis Ababa, Ethiopia. absosina2011@gmail.com.
BMC infectious diseases
|November 22, 2024
概括
研究人员确定了八种血清蛋白生物标志物,以准确诊断患者的活跃肺结核 (TB),无论潜在的结核感染状况如何. 这一发现可能会导致改善结核病控制策略.
科学领域:
- 生物化学 生物化学
- 免疫学 免疫学 免疫学
- 分子诊断学 分子诊断
背景情况:
- 对结核病 (TB) 的非吐诊断标志物非常需要.
- 这项研究旨在发现活性肺结核 (PTB) 诊断的潜在血清蛋白生物标志物.
研究的目的:
- 为了确定新的血清蛋白生物标记物,以准确诊断活性肺结核病.
- 评估已识别的生物标志物的诊断性能,以区分PTB与其他呼吸道疾病.
主要方法:
- 接近延伸试验 (PEA) 用于测量血清样本中的92个与炎症相关的蛋白质生物标志物.
- 基因本体学 (GO) 和KEGG通路分析进行了功能洞察.
- 拉索回归确定了候选生物标志物,与ROC分析的后勤回归评估了诊断性能.
主要成果:
- 与对照组相比,在活跃的PTB患者中发现了37种差异表达蛋白 (DEP).
- DEP主要参与炎症反应和细胞因子-细胞因子相互作用.
- 八种候选蛋白质生物标记物 (IFN-,LIF,uPA,CSF-1,SCF,SIRT2,4E-BP1,GDNF) 已被确定具有高诊断准确度 (AUC>0.92).这些生物标记物具有很高的诊断准确度.
结论:
- 八种蛋白质标志物有效地区分了活跃的PTB与其他呼吸道疾病,而不管是潜在的结核病感染.
- 这些标志物显示出开发用户友好,负担得起的治疗点测试的潜力,以便在高负担地区加强结核病控制.
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