Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Genome Annotation and Assembly03:36

Genome Annotation and Assembly

18.8K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
18.8K
Genome Copying Errors02:46

Genome Copying Errors

4.2K
DNA replication is a well-evolved process that copies millions of base pairs with high fidelity during each cell division. Occasionally a wrong base or a long stretch of wrong bases may get added to the daughter strands. If the errors are left unchecked, cells might accumulate several mutations that might endanger their  survival. Therefore, the copying errors are checked and repaired at three levels.
4.2K
Comparing Copy Number Variations and SNPs02:26

Comparing Copy Number Variations and SNPs

17.3K
Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
17.3K
Sanger Sequencing01:57

Sanger Sequencing

752.9K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
752.9K
Next-generation Sequencing03:00

Next-generation Sequencing

87.5K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
87.5K
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

12.3K
The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
12.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Epigenetic orchestration of sugar signaling in plant development and stress adaptation.

Horticulture research·2026
Same author

A k-mer-based genome-wide association study approach empowering gene mining in polyploids.

Nature genetics·2026
Same author

Genetic architecture of sugarcane traits in a polyploid genomics framework.

Nature·2026
Same author

Pan-genome analysis uncovers extensive structural variations in Africa's indigenous legume crop cowpea.

Nature communications·2026
Same author

Exploring the developmental mechanisms of tea plant trichomes using genomics and single-cell transcriptome sequencing.

Horticulture research·2026
Same author

Integrating GWAS, QTL Mapping, and RNA-seq to Identify Candidate Genes for Kernel Weight and Oil Traits in Maize (<i>Zea mays</i> ssp. <i>mays</i>).

Journal of agricultural and food chemistry·2026
Same journal

Direct link between convergent evolution at sequence level and phenotypic level of septal pore cap in Agaricomycotina.

G3 (Bethesda, Md.)·2026
Same journal

Experimental evolution reveals bifunctional genetic solutions to loss of trpF in Salmonella enterica.

G3 (Bethesda, Md.)·2026
Same journal

Spargel/dPGC-1 influences cell growth through the E2F1-mediated endocycle pathway.

G3 (Bethesda, Md.)·2026
Same journal

Loss of ptr-6 restores eggshell integrity and embryonic viability in C. elegans fatty acid synthase mutants.

G3 (Bethesda, Md.)·2026
Same journal

A pcyt-1 Allelic Series Reveals In Vivo Consequences of Reduced Phosphatidylcholine Synthesis in C. elegans.

G3 (Bethesda, Md.)·2026
Same journal

Copy Number Variation: A Substrate for Plant Adaptation and Stress Response in Arabidopsis.

G3 (Bethesda, Md.)·2026
查看所有相关文章

相关实验视频

Updated: Jun 6, 2025

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms
09:30

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Published on: September 13, 2018

9.5K

基于对线性的组合校正工具 GUI:基于对线性的基因组组合校正软件.

Shengcheng Zhang1,2, Hejun Du1, Xingtan Zhang2

  • 1Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang 443100, China.

G3 (Bethesda, Md.)
|November 22, 2024
PubMed
概括
此摘要是机器生成的。

基因组组装错误会影响下游分析. 基于对线性组装校正工具的GUI使用参考基因组对线性来纠正这些错误,为改进基因组准确性提供手动和自动调整.

关键词:
软件 Python 是一个 Python 软件.算法算法是一种算法.基因组组装组合的基因组.手动纠正手动纠正

更多相关视频

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

5.0K
RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord
11:13

RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord

Published on: November 1, 2014

14.6K

相关实验视频

Last Updated: Jun 6, 2025

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms
09:30

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Published on: September 13, 2018

9.5K
Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

5.0K
RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord
11:13

RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord

Published on: November 1, 2014

14.6K

科学领域:

  • 基因组学和生物信息学
  • 计算生物学 计算生物学

背景情况:

  • 基因组组装错误可能会严重损害后续基因组分析的可靠性.
  • 精确的基因组组合对于理解遗传变异,进化和疾病至关重要.

研究的目的:

  • 引入基于对线性的组装校正工具图形用户界面 (GUI) 来纠正基因组组装错误.
  • 提供一个用户友好的平台,用于手动和自动纠正大规模组装缺陷,特别是在多倍体基因组.

主要方法:

  • 利用组装基因组和参考基因组之间的对线性信息.
  • 实现一个支持手动校正操作的GUI:插入,删除,反转和交换结合体和染色体.
  • 自动重组,重新标记和重新绘制组件修改后的变化跟踪.

主要成果:

  • 该工具能够有效地可视化和纠正基因组组装错误.
  • 先进的对线性检测功能有助于识别和解决大规模组装问题.
  • 该软件支持复杂的多倍体基因组组合中的强大的错误校正.

结论:

  • 基于对线性的组装校正工具GUI是改善基因组组装质量的有效解决方案.
  • 它的用户友好的界面和先进的功能使得它对基因组研究非常有价值,特别是对多倍体生物的研究.