Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Protein Translocation Machinery on the ER Membrane01:28

Protein Translocation Machinery on the ER Membrane

4.5K
The translocon complex situated on the ER membrane is the main gateway for the protein secretory pathway. It facilitates the transport of nascent peptides into the ER lumen and their insertion into the ER membrane.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the...
4.5K
Cotranslational Protein Translocation01:20

Cotranslational Protein Translocation

7.2K
Translocation of proteins across membranes is an ancient process that occurs even in bacteria and archaebacteria. In fact, the components of the translocation machinery are still conserved between prokaryotes and eukaryotes.
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...
7.2K
Vesicular Tubular Clusters01:45

Vesicular Tubular Clusters

2.4K
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
2.4K
Rab Cascades01:25

Rab Cascades

2.6K
Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
2.6K
Nuclear Protein Sorting01:34

Nuclear Protein Sorting

4.5K
Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
4.5K
Directionality of Nuclear Transport01:42

Directionality of Nuclear Transport

3.2K
Ras-related nuclear protein or Ran is a small G protein that cycles between its GTP and GDP bound states. Ran specific regulators, a Ran GTPase Activating Protein or RanGAP present in the cytosol and a Ran guanine nucleotide exchange factor or RanGEF present inside the nucleus regulate GTP/GDP exchange. A high concentration of GTP inside the cells, in addition to this asymmetric distribution of  Ran-specific regulators, leads to a higher RanGTP concentration inside the nucleus. This...
3.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

A Hybrid Ensemble System for Time-Series Anomaly Detection in Automated Quality Control of Medical Equipment.

Diagnostics (Basel, Switzerland)·2026
Same author

Critical amino acid residues in the N-terminal domain of NADPH-dependent assimilatory sulfite reductase flavoprotein mediate octameric assembly.

Protein science : a publication of the Protein Society·2026
Same author

Crocin Targets HSP90 to Suppress NLRP3/Caspase-1-Mediated Sertoli Cell Pyroptosis.

Molecular reproduction and development·2026
Same author

Author Correction: In situ cryo-ET defines the ultrastructure of ER exit sites in human cells.

Nature cell biology·2026
Same author

EphB4 inhibition defines a druggable synthetic-lethal vulnerability in MYC-driven triple-negative breast cancer.

Cell death & disease·2026
Same author

Surface BO<sub>3</sub> Configuration in Li-Rich Cathode Materials Enabling Highly-Stable Anionic Redox Reactions.

Angewandte Chemie (International ed. in English)·2026

相关实验视频

Updated: Jun 6, 2025

Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells
11:05

Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells

Published on: February 21, 2019

9.0K

在神经元货物贩运中,TFG环复合体的多重作用

Ziheng Zhang, Molly M Lettman, Amber L Schuh

    bioRxiv : the preprint server for biology
    |November 22, 2024
    PubMed
    概括

    在Trk融合基因 (TFG) 的病理变异导致遗传性性 (HSP) 通过破坏其八米环结构. 这些突变会破坏TFG复合体的稳定,通过不同的机制导致轴突病变和神经退行.

    更多相关视频

    Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
    08:55

    Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

    Published on: December 29, 2017

    9.5K
    Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis
    11:09

    Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis

    Published on: October 30, 2014

    9.3K

    相关实验视频

    Last Updated: Jun 6, 2025

    Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells
    11:05

    Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells

    Published on: February 21, 2019

    9.0K
    Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
    08:55

    Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

    Published on: December 29, 2017

    9.5K
    Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis
    11:09

    Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis

    Published on: October 30, 2014

    9.3K

    科学领域:

    • 神经科学是一个神经科学.
    • 结构生物学 结构生物学
    • 遗传学 遗传学 是一个

    背景情况:

    • Trk融合基因 (TFG) 的病理变异与神经退行性疾病有关.
    • TFG氨基终端PB1域中的突变与遗传性性 (HSP) 有关.
    • 对于TFG在HSP中的作用的结构性基础仍然不清楚.

    研究的目的:

    • 为了阐明TFG八合体形成的结构机制.
    • 研究HSP相关突变如何影响TFG的结构和功能.
    • 了解TFG介导的神经退行症的分子基础.

    主要方法:

    • 在X射线晶体学.
    • 低温电子显微镜的使用方法
    • 结构建模 结构建模

    主要成果:

    • 一个TFG的结构模型揭示了它的八度环复合体的形成.
    • 一个由静电和水相互作用组成的网络稳定了TFG原体接口.
    • 与HSP相关的突变破坏了这种接口,破坏了八度体的稳定,导致了轴突病.
    • 在体内,TFG变异的影响不均,这表明病原性机制多样化.

    结论:

    • 通过特定的分子间相互作用,TFG形成了八米环.
    • 在TFG中引起HSP的突变会使这些八度体不稳定,从而导致神经退行.
    • 不同的机制是TFG突变引起的神经退行性疾病的基础.