米罗1表达改变了全球基因表达,ERK1/2酸化,氧化和细胞周期进展
Nathaniel Shannon1, Cory Raymond1, Chloe Palmer1
1Department of Pathology and Laboratory Medicine, University of Vermont Cancer Center, Larner College of Medicine, Burlington, VT 05405, USA.
bioRxiv : the preprint server for biology
|November 22, 2024
概括
线粒体适配器Miro1对于细胞生长和循环进展至关重要. 它的缺失破坏了线粒体的运输,导致ERK1/2信号的改变和细胞增殖缺陷.
科学领域:
- 细胞生物学 细胞生物学
- 线粒体动力学的动力学
- 分子信号传输的方法
背景情况:
- 线粒体定位对于细胞能量和信号传递至关重要.
- Miro1通过微管子运动蛋白促进了线粒体的运输.
- 线粒体中Miro1的缺失导致线粒体周核封存和破坏梯度.
研究的目的:
- 研究Miro1删除对MEF细胞生长和细胞周期的影响.
- 确定与Miro1缺乏相关的转录性变化.
- 阐明Miro1在调节ERK1/2信号通路中的作用.
主要方法:
- 对Miro1淘汰赛 (Miro1-/-) 和野生类型 (Miro1+/+) MEF的比较增长率分析.
- 使用流细胞计进行细胞周期分析.
- RNA测序以评估Miro1-/- MEFs中的基因表达变化.
- 西方涂抹和质谱法用于评估蛋白质酸化和氧化状态.
主要成果:
- Miro1-/- MEFs表现出较慢的生长和细胞周期缺陷 (减少G1,增加S阶段).
- RNA测序揭示了细胞增殖,迁移和MAP激酶信号基因的差异表达.
- 在Miro1-/- MEF中,ERK1/2酸化升高;DUSP1-6表达和氧化保持不变.
- 在Miro1-/- MEF中观察到ERK1/2氧化的增加.
结论:
- Miro1对于正常的细胞增殖和细胞周期进展至关重要.
- 米罗1缺乏影响转录调节和MAPK信号,特别是ERK1/2.2.
- 在Miro1-/- MEFs中改变ERK1/2信号和氧化可能是观察到的细胞缺陷的基础.
相关概念视频
mTOR Signaling and Cancer Progression
3.7K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.7K
MAPK Signaling Cascades
5.2K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
5.2K
PI3K/mTOR/AKT Signaling Pathway
3.4K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.4K
M-Cdk Drives Transition Into Mitosis
5.5K
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
5.5K
Interactions Between Signaling Pathways
6.2K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.2K
Mitogens and the Cell Cycle
6.4K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.4K


