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相关概念视频

Notch Signaling Pathway03:14

Notch Signaling Pathway

4.2K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
4.2K
Neurulation01:30

Neurulation

41.7K
Neurulation is the embryological process which forms the precursors of the central nervous system and occurs after gastrulation has established the three primary cell layers of the embryo: ectoderm, mesoderm, and endoderm. In humans, the majority of this system is formed via primary neurulation, in which the central portion of the ectoderm—originally appearing as a flat sheet of cells—folds upwards and inwards, sealing off to form a hollow neural tube. As development proceeds, the...
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Determination01:51

Determination

18.2K
During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
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Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

7.2K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
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相关实验视频

Updated: Jun 6, 2025

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis
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Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Published on: December 12, 2019

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条形码 在发育中的大脑中发出痕信号.

Abigail M Siniscalco1, Roshan Priyarangana Perera1, Jessie E Greenslade1

  • 1Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA.

Development (Cambridge, England)
|November 22, 2024
PubMed
概括
此摘要是机器生成的。

我们开发了SABER-seq,这是一个基于CRISPR的工具,可以永久记录发育信号事件,如Notch信号. 这允许精确跟踪细胞系及其信号历史在斑马鱼大脑的发展.

关键词:
条形码编码 条形码编码大脑发育 大脑发育大脑的scRNA-seqq.克里斯普尔是什么意思?克里斯普尔是什么意思?神经新生是神经发生的过程.口信号传输是没有的.

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Antibody Uptake Assay for Tracking Notch/Delta Endocytosis During the Asymmetric Division of Zebrafish Radial Glia Progenitors
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Placing Growth Factor-Coated Beads on Early Stage Chicken Embryos
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Placing Growth Factor-Coated Beads on Early Stage Chicken Embryos

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相关实验视频

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Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis
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Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

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Antibody Uptake Assay for Tracking Notch/Delta Endocytosis During the Asymmetric Division of Zebrafish Radial Glia Progenitors
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Antibody Uptake Assay for Tracking Notch/Delta Endocytosis During the Asymmetric Division of Zebrafish Radial Glia Progenitors

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Placing Growth Factor-Coated Beads on Early Stage Chicken Embryos
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Placing Growth Factor-Coated Beads on Early Stage Chicken Embryos

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科学领域:

  • 发展生物学 发展生物学
  • 遗传学 遗传学 是一个
  • 神经科学是一个神经科学.

背景情况:

  • 发育信号通路指导细胞命运的决定和行为.
  • 现有的光记者缺乏细胞类型的可扩展性和分子分辨率.
  • 了解瞬态信号动态对于绘制发育过程至关重要.

研究的目的:

  • 介绍SABER-seq,一种新的CRISPR-Cas分子记录器,用于捕获短暂的发育信号.
  • 为了证明SABER-seq在记录斑马鱼大脑发育中的Notch信号的能力.
  • 根据历史信号活动,实现细胞系的高分辨率映射.

主要方法:

  • SABER-seq使用了CRISPR-Cas系统,其中包括一个信号传感器和一个条形码记录器.
  • 传感器以依赖口的,可诱导的方式激活Cas9.
  • 突变被永久存储在基因组中,允许通过单细胞转录学进行后期分析.

主要成果:

  • SABER-seq成功地记录了斑马鱼大脑发育中的Notch信号动态.
  • 在与大脑图谱相结合时,SABER-seq识别了来自Notch-active祖先的细胞类型.
  • 在成熟的神经元亚型中检测到祖先中差异性Notch活动的罕见实例.

结论:

  • SABER-seq提供了一个可扩展的,高分辨率的平台,用于绘制发展信号.
  • 这项技术允许在后来的发育阶段解构短暂的信号事件.
  • SABER-seq增强了我们追踪细胞系和理解信号在分化中的作用的能力.