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Updated: Jun 6, 2025

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Published on: December 12, 2019

条形码在发育中的大脑中发出痕信号

Abigail M Siniscalco¹, Roshan Priyarangana Perera¹, Jessie E Greenslade¹

¹Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA.

Development (Cambridge, England)

|November 22, 2024

概括

我们开发了SABER-seq,这是一个基于CRISPR的工具,可以永久记录发育信号事件,如Notch信号. 这允许精确跟踪细胞系及其信号历史在斑马鱼大脑的发展.

科学领域:

发展生物学发展生物学
遗传学遗传学是一个
神经科学是一个神经科学.

背景情况:

发育信号通路指导细胞命运的决定和行为.
现有的光记者缺乏细胞类型的可扩展性和分子分辨率.
了解瞬态信号动态对于绘制发育过程至关重要.

研究的目的:

介绍SABER-seq,一种新的CRISPR-Cas分子记录器,用于捕获短暂的发育信号.
为了证明SABER-seq在记录斑马鱼大脑发育中的Notch信号的能力.
根据历史信号活动,实现细胞系的高分辨率映射.

主要方法:

SABER-seq使用了CRISPR-Cas系统,其中包括一个信号传感器和一个条形码记录器.
传感器以依赖口的,可诱导的方式激活Cas9.
突变被永久存储在基因组中,允许通过单细胞转录学进行后期分析.

主要成果:

SABER-seq成功地记录了斑马鱼大脑发育中的Notch信号动态.
在与大脑图谱相结合时,SABER-seq识别了来自Notch-active祖先的细胞类型.
在成熟的神经元亚型中检测到祖先中差异性Notch活动的罕见实例.

结论:

SABER-seq提供了一个可扩展的,高分辨率的平台,用于绘制发展信号.
这项技术允许在后来的发育阶段解构短暂的信号事件.
SABER-seq增强了我们追踪细胞系和理解信号在分化中的作用的能力.

关键词:

条形码编码条形码编码大脑发育大脑发育大脑的scRNA-seqq.克里斯普尔是什么意思?克里斯普尔是什么意思?神经新生是神经发生的过程.口信号传输是没有的.

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