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转录因子GmERFVII上调与PATHOGENESIS-RELATED10相关的调节,并有助于大豆囊线虫耐药性

Miaomiao Deng¹, Lei Zhang¹, Chao Yang¹

¹National Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.

Plant physiology

|November 22, 2024

概括

大豆囊线虫感染会触发类似缺氧的反应,激活ERFVII转录因子. 这些因素通过调节防御基因来增强大豆的耐药性,从而加强对病原体的免疫力.

科学领域:

植物分子生物学植物分子生物学
植物病原体相互作用
无生物和生物应激反应的应激反应.

背景情况:

第七组乙烯反应因子 (ERFVII) 转录因子调节植物对低氧 (低氧) 的反应.
它们在生物应激中的作用,特别是在大豆与虫相互作用中的作用,尚不清楚.
大豆囊线虫 (SCN) 是影响大豆生产的主要病原体.

研究的目的:

在SCN感染期间调查大豆ERFVII转录因子的功能和调节机制.
阐明ERFVIIs在大豆防御SCN中的作用.

主要方法:

在SCN感染下ERFVII蛋白稳定和基因表达的分析.
缺氧预处理实验,以评估其对线虫耐药性的影响.
转基因毛发根分析,以评估GmERFVIIs和N-end规则通路组件的功能.

主要成果:

SCN感染诱导大豆的缺氧类反应,包括ERFVII稳定和增加缺氧反应基因的表达.
低氧预处理增强了大豆对SCN的抵抗力.
GmERFVII (GmRAP2.12,GmRAP2.3) 激活GmPR10-09g的表达,这是一个积极影响线虫耐药性的基因.
在大豆防御SCN方面,ERFVII发挥了积极的作用.

结论:

大豆ERFVIIs是对SCN的防御反应的关键调节者.
通过转录激活GmPR10,GmERFVII增强了大豆免疫力,突出显示了低氧反应和生物应激防御之间的联系.

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