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肉GSDME,一个主要的孔形成分子,负责RNA病毒诱导的肉中火灭菌

Zhi Chen^1,2,3, He Chang^1,2,3, Shujun Zhang^1,2,3

¹National Key Laboratory of Veterinary Public Health Security, Beijing, China.

Journal of virology

|November 22, 2024

概括

由于GSDMD缺乏,炎症性细胞死亡的火,是难以捉摸的. 研究人员发现RNA病毒通过chGSDME触发细胞死亡,进步对宿主防御机制对病毒感染的理解.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
病毒学病毒学

背景情况:

热是关键的炎症编程细胞死亡途径.
加斯德明D (GSDMD) 对于哺乳动物的热致死是必不可少的.
鸟类热的机制,特别是在中,仍然不太了解.

研究的目的:

为了阐明RNA病毒感染期间中热的机制.
为了确定参与火灭的关键分子参与者.
了解加斯德明家族蛋白质在禽类抗病毒反应中的作用.

主要方法:

的DF-1细胞系感染了传染病病毒 (IBDV) 和其他RNA病毒 (VSV,AIV,NDV).
对细胞死亡,chGSDME裂变和病毒释放的分析.
使用了MDA5介导的信号通路激活和Poly (I:C) 处理.
在chGSDME表达中采用了淘汰和淘汰策略.

主要成果:

IBDV和其他RNA病毒诱导细胞死亡,包括GSDME (chGSDME) 在DF-1细胞中的裂变.
激活了MDA5信号通路,导致chCaspase-3/7介导的chGSDME在270DAVD273.3位点上的裂变.
chGSDME缺乏症显著降低IBDV诱导的热和病毒释放.

结论:

在RNA病毒感染期间,肉中毒是由chMDA5-CASP8/9-CASP3/7-chGSDME途径介导的.
chGSDME在的抗病毒防御中发挥着至关重要的作用,通过促进热和限制病毒传播.
这些发现为Gasdermin家族蛋白在物种间宿主-病原体相互作用中的多样性作用提供了洞察力.

关键词:

在GSDME中,GSDME是GSDME.在IBDV中,IBDV是指IBDV.卡斯帕斯-3酶是什么意思在Caspase-7中使用.热致灭 (pyroptosis) 是一种致的过程.

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