ARPP19酸化位点的演变以及脊椎动物卵细胞成熟的cAMP控制中的切换
Ferdinand Meneau1, Pascal Lapébie2, Enrico Maria Daldello1
1Sorbonne Université, CNRS, Laboratoire de Biologie du Développement - Institut de Biologie Paris Seine, LBD - IBPS, F-75005 Paris, France.
概括
矛盾的是,循环AMP-蛋白激酶A (cAMP-PKA) 信号传递对卵细胞成熟有影响. 在Clytia中,Gwl的ARPP19酸化促进了成熟,但PKA的不良识别限制了它的作用,与脊椎动物不同.
科学领域:
- 细胞和发育生物学
- 分子信号传输的方法
- 进化生物学 进化生物学
背景情况:
- 循环AMP-蛋白激酶A (cAMP-PKA) 信号传递在动物种群中对卵细胞成熟起着双重作用.
- 这种信号通路在许多物种中启动成熟,但在脊椎动物中抑制它,呈现出"cAMP悖论".
- 蛋白质ARPP19是该途径的关键基质,特别是在脊椎动物中.
研究的目的:
- 通过检查脊椎动物 (Xenopus) 和类动物 (Clytia) 中ARPP19的酸化和功能来研究"cAMP悖论".
- 为了比较ARPP19在不同动物血统的卵细胞成熟过程中的功能.
主要方法:
- 在Xenopus和Clytia之间交换ARPP19蛋白质.
- 在体内和体外分析ARPP19酸化模式.
- 评估外源ARPP19对卵细胞成熟的功能影响.
主要成果:
- 克莱蒂亚卵细胞在一个保存的Gwl位点上表现出ARPP19酸化,抑制PP2A并促进M相进入,类似于Xenopus.
- 尽管保留了PKA动机,但Clytia ARPP19在体外和体内都显示了PKA的低酸化.
- 外源的Clytia ARPP19没有延迟Xenopus卵细胞的成熟,与Xenopus ARPP19.
结论:
- 在Clytia中,ARPP19不能调节卵细胞成熟的启动,这是由于PKA的识别能力差,以及缺乏脊椎动物特异性的效应因子.
- 祖先的ARPP19可能通过Gwl酸化保留了保存的M相作用.
- 后来ARPP19的增强PKA酸化进化,使其能够参与脊椎动物的卵细胞成熟调节.
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