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相关概念视频

Plant Tissue Culture02:57

Plant Tissue Culture

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Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.
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Chromatin Immunoprecipitation- ChIP02:36

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Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
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Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
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Plants have rigid cell walls that are made up of cell wall polysaccharides that mediate cell-cell adhesion. The primary cell walls of plants consist of two independent and interacting polysaccharide networks: a pectin matrix that embeds the second network comprising cellulose and hemicelluloses.
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Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
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相关实验视频

Updated: Jun 6, 2025

Detection of Histone Modifications in Plant Leaves
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植物组织的HiChIP.

Rim Brik Chaouche1, Cécile Raynaud1, Moussa Benhamed1

  • 1Institute of Plant Sciences-Paris-Saclay, Gif-sur-Yvette, France.

Methods in molecular biology (Clifton, N.J.)
|November 22, 2024
PubMed
概括
此摘要是机器生成的。

本研究详细介绍了一种使用HiChIP分析植物三维 (3D) 基因组组织的方法. 这项技术捕捉了染色体相互作用,提供了对基因组折叠和转录调节的全面视图.

关键词:
染色体的形状是如何形成的在HiChIP中,您可以使用HiChIP.植物 植物 植物

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Time-lapse Fluorescence Imaging of Arabidopsis Root Growth with Rapid Manipulation of The Root Environment Using The RootChip
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科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

  • 表观遗传学传统上使用线性基因组模型,但3D染色质折叠对于转录调节至关重要.
  • 与测序相结合的近距离结合技术可以研究长距离/短距离染色体相互作用和3D基因组组织.
  • HiChIP提供了一个以蛋白质为中心的方法,用于全面的染色体接触分析.

研究的目的:

  • 为生成HiChIP库提供详细的协议.
  • 为植物组织适应HiChIP方法.
  • 促进研究3D染色体组织及其在植物基因调节中的作用.

主要方法:

  • 准备HiChIP库的详细协议.
  • 在植物组织中应用HiChIP.
  • 高通量测序用于分析色素相互作用.

主要成果:

  • 从植物样本成功生成了HiChIP库.
  • 提供了有关特定因素的染色质接触的全面视图.
  • 能够对植物的3D基因组结构进行详细分析.

结论:

  • 描述的HiChIP程序对植物组织有效.
  • 这种方法增强了对植物3D基因组组织和转录调节的理解.
  • HiChIP是植物表观遗传学研究的强大工具.