在植物核中同时可视化蛋白质和基因组区域
Methods in molecular biology (Clifton, N.J.)
|November 22, 2024
概括
这项研究引入了一种结合光现场杂交 (FISH) 和免疫组织化 (IHS) 方法,用于同时可视化植物核中的蛋白质和基因组位点. 这种技术增强了对核动力学和空间关系的理解.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 免疫组织化 (IHS) 使用抗体检测细胞/组织中的蛋白质.
- 光在位杂交 (FISH) 可视化基因组区域和使用寡核酸探针的核定位.
研究的目的:
- 描述一种FISH-IHS组合技术,用于同时对植物核中的蛋白质和基因组位置进行可视化.
- 为了研究核动力学和蛋白质和DNA之间的空间关系.
主要方法:
- 开发一个结合的FISH-IHS协议.
- 对于提取的植物核的应用.
- 适用于嵌入氨酸的植物组织部分.
主要成果:
- 在植物核中同时检测和定位特定的蛋白质和基因组位置.
- 证明该方法对单独的核和组织部分的适用性.
结论:
- 结合FISH-IHS技术是植物核研究的一个有价值的工具.
- 它有助于更深入地了解植物核中的空间组织.
更多相关视频
09:33An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis
Published on: June 19, 2014
13.1K
13:06Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging ESI
Published on: September 24, 2015
10.1K
相关概念视频
The Nucleus
The nucleus is a membrane-bound organelle that acts as a control center in a eukaryotic cell. It contains chromosomal DNA, which controls gene expression and precisely regulates the production of proteins within the cell. In contrast, the DNA inside the mitochondria and chloroplast only carries out functions that are specific to those organelles.
Arrangement of DNA within Nucleus
The regulation of gene expression inside the nucleus is dependent on many factors, including the DNA structure. The...
Arrangement of DNA within Nucleus
The regulation of gene expression inside the nucleus is dependent on many factors, including the DNA structure. The...
In-situ Hybridization
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
Transgenic Plants
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Animal and Plant Cell Structure
Animal and plant cells not only differ in their structure, function, and mode of nutrition but also in how they reproduce, specialize, and organize into complex structures.
Cell Division
Though both plant and animal cells divide by mitosis (for non-gametic cells) and meiosis (for gametic cells), they differ in the specifics of this process. Unlike animal cells, plant cells lack centrosomes — an organelle responsible for organizing the spindle fibers and segregating the chromosomes during cell...
Cell Division
Though both plant and animal cells divide by mitosis (for non-gametic cells) and meiosis (for gametic cells), they differ in the specifics of this process. Unlike animal cells, plant cells lack centrosomes — an organelle responsible for organizing the spindle fibers and segregating the chromosomes during cell...
The Nucleus
The nucleus is a membrane-bound organelle that acts as a control center in a eukaryotic cell. It contains chromosomal DNA, which controls gene expression and precisely regulates the production of proteins within the cell. In contrast, the DNA inside the mitochondria and chloroplast only carries out functions that are specific to those organelles.
Arrangement of DNA within Nucleus
The regulation of gene expression inside the nucleus is dependent on many factors, including the DNA structure. The...
Arrangement of DNA within Nucleus
The regulation of gene expression inside the nucleus is dependent on many factors, including the DNA structure. The...
Plant Tissues
Plants are multicellular eukaryotes with tissue systems made of various cell types that carry out specific functions. Different tissues work together to perform a unique function and form an organ. Organs working together form organ systems. Vascular plants have two distinct organ systems: a shoot system and a root system. The shoot system consists of two portions: the vegetative (non-reproductive) parts of the plant, such as the leaves and the stems, and the reproductive parts of the plant,...
