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子宫内膜细胞外囊泡调节与胚胎发育和人类胚胎囊植入相关的过程

Marina Segura-Benítez^1,2, Maria Cristina Carbajo-García^1,2, Alicia Quiñonero¹

¹Fundación IVI, Instituto de Investigación Sanitaria La Fe, Valencia, Spain.

Human reproduction (Oxford, England)

|November 22, 2024

概括

孕产妇子宫内膜外细胞囊泡 (EVs) 显著改变了人体胚胎细胞的基因表达,影响了对胚胎发育和植入至关重要的途径. 这揭示了影响早期怀孕的关键通信系统.

科学领域:

生殖生物学和胚胎学
细胞和分子生物学细胞和分子生物学
基因组学和转录基因组学

背景情况:

细胞外囊泡 (EVs) 中介细胞间通信,子宫内膜EVs参与调节胚胎植入.
以前的研究表明子宫内膜EVs含有影响植入的miRNA和蛋白质,但它们对人类胚胎转录组的影响尚不清楚.

研究的目的:

研究人类胚胎囊细胞在内化由人类子宫内膜分泌的细胞外囊泡 (EVs) 时的转录基因反应.

主要方法:

培养了原始人类子宫内膜上皮细胞以分离EVs.
第五天,人类胚胎囊与或没有这些EVs培养了24小时.
进行RNA测序以分析基因表达变化,识别差异表达基因 (DEG) 和执行途径分析.

主要成果:

EVs诱导了胚胎细胞基因表达的显著变化,519个基因上调和395个基因下调.
调节后的DEG被丰富了与胚胎发育,入侵,细胞循环和活力相关的功能.
下调的DEG与细胞死亡和DNA碎片化有关;参与多能性和植入的关键途径被调节,子宫内膜EV小RNA向大约80%的DEG.

结论:

子宫内膜EVs在人体胚胎囊中触发了转录基因反应,调节了对发育和植入至关重要的分子机制.
这项研究强调了EV介导通信和EV-miRNA调节在早期胚胎发生过程中的功能意义.
研究结果表明,潜在的诊断生物标志物可以预测植入成功.

关键词:

在RNA-seqqq.胚胎植入胚胎的植入这是子宫内膜细胞的细胞.细胞外囊泡中的细胞外囊泡.人类胚胎囊在 miRNA 调节过程中.翻译学翻译学翻译学翻译学

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