二次性酒精的二酶动态动态动力学分辨率通过在水中的化/拉塞米化
Aleksandra Rudzka1, Tamara Reiter2, Wolfgang Kroutil2,3
1Laboratory of Biocatalysis and Biotransformation, Department of Drugs Technology and Biotechnology, Faculty of Chemistry, Warsaw University of Technology, Koszykowa 75, 00-662, Warsaw, Poland.
这项研究引入了一种新型的水态动态动力学分辨率 (DKR),用于使用两个酶的raceme sec-alcohols. 这种化学酶方法在水中实现了高产量和光学纯度,克服了以前的局限性.
科学领域:
- 生物催化剂是一种生物催化剂.
- 有机化学 有机化学
- 绿色化学 绿色化学
背景情况:
- 动态动力学分辨率 (DKR) 对于从racemic混合物中合成光学纯化合物至关重要.
- 对于二次醇的现有DKR方法通常依赖于有机溶剂和多种催化剂.
- 在水溶液中执行DKR存在重大挑战.
研究的目的:
- 开发一种化学酶式的DKR工艺,用于完全在水溶液中溶解的血糖干酒.
- 使用最小数量的生物催化剂来实现高产量和酶选择性.
- 建立一个更绿色,更有效的方法,用于合性酒精合成.
主要方法:
- 使用非立体选择性酒精脱酶 (Lk-ADH-Prince) 通过借用快速种族化.
- 在水中采用工程化乙转移酶变体 (MsAcT) 进行反选择性乙化.
- 确定了2,2,2-trifluoroethyl乙酸盐作为一种有效的乙捐赠剂.
主要成果:
- 在水性缓冲体中证明了各种raceme (hetero) 醇的成功DKR.
- 通过使用 (R) 选择性 MsAcT 实现了 (R) 乙酸盐的高转换率 (>99%) 和优异的光学纯度 (83-99.9% ee).
- 使用立体补充性 (S) 选择性MsAcT变体获得的 (S) - 酸盐.
- 在某些情况下,对高基质度 (高达400mM) 的耐受性.
结论:
- 开发了第一个仅用两个酶在水溶液中的sec-alcohols的DKR.
- 这种方法为传统的DKR协议提供了可持续和高效的替代方案.
- 该系统具有多功能性,可以获得高纯度的 (R) 和 (S) 双反体.
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