在子肌纤维蛋白中,糖化诱导的修饰的结构和功能影响
Ruiqi Cao1, Bangxu Wang1, Ting Bai1
1Meat Processing Key Laboratory of Sichuan Province, College of Food and Biological Engineering, Chengdu University, Chengdu 610106, PR China.
International journal of biological macromolecules
|November 22, 2024
概括
糖化增强了子的肌纤维蛋白质,提高了溶解性,抗氧化能力和热稳定性. 这种修改为开发高质量,营养丰富的肉产品提供了有希望的途径.
科学领域:
- 食品科学 食品科学 食品科学
- 蛋白质化学 蛋白质化学
- 生物化学 生化学
背景情况:
- 子肉在营养上有价值,但其肌纤维蛋白功能不佳,影响产品质量.
- 蛋白质修饰对于克服食品加工中的这些局限性至关重要.
- 糖化是改善蛋白质功能性质的有效策略.
研究的目的:
- 为了研究糖化对子肌纤维蛋白的影响.
- 评估不同糖化剂 (葡萄糖,乳糖,β-葡萄糖) 对蛋白质功能的影响.
- 为了理解由糖基化诱导的构造变化和分子相互作用.
主要方法:
- 用葡萄糖,乳糖和β-葡萄糖对子肌纤维蛋白进行糖化修饰.
- 评估蛋白质溶解性,降解能力和抗氧化活性 (•OH和DPPH基因清除).
- 分析蛋白质的热稳定性和形状变化 (热变性,光,水性).
- 分子对接模拟以阐明相互作用机制.
主要成果:
- 甘化显著提高了蛋白质溶解度 (高达34%) 和降解能力.
- 观察到增强的抗氧化性质,OH基因清除率增加到94.21%,DPPH基因清除率增加到76.21%.
- 糖化增加了热稳定性,改变了蛋白质构造 (光,疏水性),分子对接证实了键和范德瓦尔斯力相互作用.
结论:
- 糖化有效地增强了子肌纤维蛋白的功能性质.
- 这种修改导致更好的溶解性,抗氧化活性和热稳定性.
- 甘化是一种可行的策略,用于生产高质量,稳定和营养丰富的子肉制品.
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