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人类加速了Shewanella oneidensis MR-1和Methanosarcina barkeri之间的物种间电子转移

Ningli Zhou¹, Dan Chen¹, Zhixing Xiao¹

¹College of Urban Construction, Nanjing Tech University, Nanjing 211816, PR China.

The Science of the total environment

|November 22, 2024

概括

非导电性湿材料 (HM) 加快了微生物物种间电子转移 (IET) 在Shewanella oneidensis MR-1和Methanosarcina barkeri.之间. 这种性物质可以提高乙醇消耗和甲生产,提供一种新的生物能源战略.

科学领域:

微生物学微生物学
环境科学环境科学
生物技术是生物技术.

背景情况:

关于物种间电子转移 (IET) 固体氧化还原介质的研究主要集中在导电材料上.
非导电性基材料 (HM) 已被事地与改善的甲基生成联系在一起,但加速IET的机制仍然未被证明.

研究的目的:

调查湿地沉积物来源的湿物质 (HMWS) 是否可以在Shewanella oneidensis MR-1和Methanosarcina barkeri之间加速IET.
阐明HMWS在促进合成微生物相互作用和增强生物能源生产中的作用.

主要方法:

建立了S. oneidensis MR-1和M. barkeri的共同培养,以乙醇作为唯一的电子捐赠者.
添加了HMWS以评估其对乙醇消费,甲性率和整体IET的影响.
确定了HMWS和参与HMWS介导IET的微生物电子载体的功能组.

主要成果:

添加HMWS使乙醇消耗增加了1.6倍,甲生成率增加了2.9倍.
在M. barkeri和S. oneidensis MR-1之间确定了一种新的HMWS介导的IET途径,它与物种间的酸盐转移不同.
在HMWS的存在下,IET总率是2.2倍高,达到0.53±0.1 meq/d·g细胞干重).
在HMWS中的CO/CO和NO/NH2功能组以及微生物黄/细胞染色体c对于HMWS介导的IET至关重要.

结论:

非导电性HMWS有效地加速微生物IET,增强合成菌-古生物学合作.
HMWS为电子转移提供了一条新的途径,显著提高了甲的性能.
这项研究提供了对自然系统中微生物电子转移的关键见解,并提出了有效的可再生生物能源生产的有希望的战略.

关键词:

胡明胡明的人类两种物种之间的电子转移.甲产生的速度是多少甲沙辛酸的 barkeri 甲沙辛酸的 barkeri 这种植物是Shewanella oneidensis MR-1

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