相关实验视频

Updated: Jun 6, 2025

Accessing the Cytotoxicity and Cell Response to Biomaterials

Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

在炎症的芽细胞中,外周溶酶体的定位促进了矿化

Nuo Xu¹, Qian Gao¹, Chengcan Yang¹

¹State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, China.

Journal of endodontics

|November 22, 2024

概括

炎症导致 lysosomes 移动到细胞边缘在 odontoblasts,促进牙矿化通过外细胞和mTORC1信号.

科学领域:

细胞生物学细胞生物学
牙科研究牙科研究
矿化过程矿化过程

背景情况:

牙细胞,负责牙形成的细胞,依赖于 lysosomal 功能进行细胞维护.
溶解体越来越多地被认为是超越降解的角色,包括通过贩运积极参与细胞反应.
了解 lysosome 动态对于理解 odontoblast 功能至关重要,特别是在诸如炎等病理条件下.

研究的目的:

为了研究溶酶体定位如何影响牙细胞矿化.
阐明了链接 lysosomal 定位和炎炎症在 odontoblasts 的机制.

主要方法:

人类牙纸干细胞分化为类似于口腔细胞的细胞 (hOBLC),并暴露在脂多糖 (LPS) 中以模拟炎.
免疫光染色被用于可视化细胞和组织中的 lysosomal 定位.
通过基因淘汰或过度表达 (SNAPIN,ARL8B) 实验改变了 lysosomal 定位,并通过 Alizarin Red S 染色和基因表达 (DSPP,DMP1) 评估了矿化.

主要成果:

在牙和LPS治疗的hOBLCs中的odontoblasts中,外围 lysosomal定位显著升高.
在体内和体外的口腔细胞分化过程中,也观察到溶酶体的这种外围转移.
在炎症的hOBLC中促进矿化与外围 lysosomal 定位有关,可能由 mTORC1 信号通路和 lysosomal exocytosis 中介.

结论:

性炎症诱导了质细胞溶解体从周核区域移动到细胞外围.
这种溶解体的再分配增强了炎症的牙细胞中的矿化.
这个过程似乎涉及mTORC1信号和溶酶体外细胞.

关键词:

lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis 是一种发生在细胞体内发生的细胞体中发生的细胞体中发生的细胞体 lysosomal 定位的位置.在 mTORC1 的情况下,mTORC1 是矿物化的矿化.耳鼻细胞质细胞.

更多相关视频

Author Spotlight: Comparing Alveolar and Long Bone Remodeling to Explore OTM Model Potential

Author Spotlight: Comparing Alveolar and Long Bone Remodeling to Explore OTM Model Potential

Published on: July 21, 2023

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis

Published on: June 24, 2018

相关概念视频

Osteoclasts in Bone Remodeling

Osteoclasts in Bone Remodeling

Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...

Bone Remodeling

Bone Remodeling

Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.