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克洛西安丁的暴露会通过线粒体氧化损伤诱导细胞亡

Wei-Long Cheng¹, Zhi-Hui Zhang¹, Zhi-Bin Zhang¹

¹School of Food and Health, Beijing Technology and Business University, Beijing, China.

Environmental toxicology

|November 23, 2024

概括

克洛西安丁 (CLO) 农药暴露会诱导人类肠道细胞的亡. 这种新型类毒素通过增加氧化应激和抑制代谢酶CYP3A4来损害细胞,从而对肠道健康构成风险.

科学领域:

毒理学毒理学毒理学
细胞生物学细胞生物学
环境健康环境健康

背景情况:

克洛西安丁 (CLO) 是一种经常在农产品中发现的新烟类农药.
农药暴露的安全性,特别是关于肠道健康的安全性,需要进行彻底的调查.

研究的目的:

为了评估克洛西安丁 (CLO) 对Caco-2细胞的亡毒性.
阐明CLO诱导的细胞损伤的潜在机制,包括氧化应激和代谢酶相互作用.

主要方法:

卡科-2细胞暴露于CLO的不同度 (100nM,10μM,1mM).
分析了与亡相关的信号蛋白 (caspases,cytochrome C),线粒体膜潜力,活性氧物种 (ROS),细胞内 (Ca2+) 和CYP3A4水平.
进行了分子对接模拟,以评估CLO与CYP3A4.4的结合.

主要成果:

暴露于CLO诱导了亡,证实了caspase 3,分离-caspase 3和caspase 9中的变化.
CLO降低了线粒体膜潜力,增加了细胞染色体C释放,ROS和Ca2+水平.
CLO降低了CYP3A4的调节,分子对接表明了直接的结合相互作用.

结论:

克洛西安丁通过氧化应激和线粒体损伤诱导Caco-2细胞的亡.
通过CLO抑制CYP3A4可能会导致其毒性.
暴露于CLO是人类肠道健康的潜在风险因素.

关键词:

灭症 (apoptosis) 是一种死亡的过程.克洛西安伊丁丁的使用方法细胞染色体P450 3A4 4A4 细胞染色体P450 3A4 4A4 细胞染色体线粒体损伤可能导致线粒体损伤.氧化应激是一种氧化应激.

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