一个重复含有的真菌效应因子通过类似于基因酶1的可塑性蛋白质重新定位,阻止其毒性
Hanqiao Liu1,2, Wenshu Zhang1,2, Qinqfei He1,2
1State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing, 210095, China.
Nature communications
|November 23, 2024
概括
研究人员在Verticillium dahliae.e.中发现了一种有毒的真菌效应物,V. dahliae四分重复蛋白 (VdTRP). 提高棉花的质量
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 农作物科学 农作物科学
背景情况:
- Verticillium dahliae通过枯病造成了大量的作物损失,特别是棉花.
- 效应剂通过与宿主细胞相互作用,在病原体毒性中起着至关重要的作用.
- 了解效应器功能和宿主目标是开发抗病策略的关键.
研究的目的:
- 为了识别和描述来自Verticillium dahliae的新型真菌作用者.
- 为了阐明确定效应器的作用机制,VdTRP.
- 探索增强农作物抵抗Verticillium wilt.的策略.
主要方法:
- 在Verticillium dahliae.中识别和结构分析VdTRP效应器.
- 使用分子试验,研究VdTRP的膜向和毒性功能.
- 产生和分析过度表达宿主标蛋白CTL1.1的转基因棉花系.
主要成果:
- 确定了一种新型的效应体,V. dahliae四重复蛋白 (VdTRP),具有CFEM域和富含proline的并列重复区域.
- VdTRP的毒性取决于其膜向,由CFEM域介导.
- 发现棉花的细胞增生蛋白,基因酶类1 (CTL1),可重定向VdTRP,其过度表达显著增强了棉花对V. dahliae的抵抗力.
结论:
- VdTRP是一种细胞有毒的真菌效应剂,其毒性依赖于膜局部.
- 操纵宿主目标蛋白CTL1提供了一种可行的策略,以赋予棉花对Verticillium枯的耐药性.
- 针对效应体与宿主相互作用,为开发耐久性作物抵抗真菌病原体提供了一个有希望的途径.
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