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淋巴细胞性冠状腺炎 arenavirus利用细胞间的连接进行细胞间的传播
Owen Byford1,2, Amelia B Shaw1,2, Hiu Nam Tse1,2
1School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK.
Scientific reports
|November 23, 2024
概括
亚里纳病毒,如淋巴细胞胆膜炎亚里纳病毒 (LCMV),通过直接的细胞对细胞接触通过纳米管状的连接传播. 这种细胞间传播途径绕过了传统的细胞外传播途径,揭示了复杂的病毒传播机制.
科学领域:
- 病毒学 病毒学
- 细胞生物学 细胞生物学
- 传染性疾病 传染性疾病
背景情况:
- 包括拉萨和LCMV在内的Arenaviridae病毒会导致严重的出血发烧和神经疾病.
- 淋巴细胞冠状腺炎的竞技病毒 (LCMV) 是一个模型的竞技病毒,用于研究病毒生物学和病变.
- 以前的理解表明,竞技病毒的传播主要通过细胞外路线发生.
研究的目的:
- 调查竞技病毒,特别是LCMV的替代传播途径.
- 确定细胞间连接在LCMV传播中的作用.
- 阐明亚雷纳病毒细胞对细胞传播的基础机制.
主要方法:
- 使用了感染LCMV的培养细胞.
- 使用共聚焦和STED显微镜可视化细胞间连接中的病毒组件.
- 使用LCMV中和抗体阻止细胞外传播和量化细胞间传播.
主要成果:
- 确定了LCMV感染细胞和未感染细胞之间富含actin和tubulin的纳米管状连接.
- 在这些细胞间连接中观察到LCMV病毒组件和基因组RNA.
- 证明,当细胞外传播被阻止时,大约50%的LCMV传播通过这些细胞间连接发生.
结论:
- 包括LCMV在内的竞技病毒利用细胞间连接,类似于道纳米管 (TNT),进行传播.
- 病毒传播比以前认为的要复杂得多,涉及细胞外和直接细胞间的途径.
- 了解这些新型传播途径对于开发有效的抗病毒策略来对抗竞技病毒感染至关重要.
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