由ABA激活的低纳米级Ca2+-CPK信号控制着根盖循环的可塑性和应激适应
Ziwei Lin1, Ying Guo1, Ruiyuan Zhang1
1State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest Agriculture & Forestry University, Yangling, China.
Nature plants
|November 23, 2024
概括
酸 (ABA) 在植物细胞中触发低纳米信号,揭示了对应激适应的新见解. 这种ABA--蛋白激酶通路在环境挑战期间调节根盖可塑性.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 细胞信号传递 细胞信号传递
背景情况:
- 酸 (ABA) 对于植物适应压力,生长和繁殖至关重要.
- 了解ABA诱导的 (Ca2+) 信号动态是有限的,特别是在防护细胞之外.
- 以前的方法很难有效地图像低纳米Ca2+变化.
研究的目的:
- 在各种植物器官和细胞类型中可视化ABA触发的Ca2+动态.
- 为了阐明与ABA信号相关的时空Ca2+特征.
- 研究ABA-Ca2+-SnRK2-CPK信号传导在根盖发育和应激反应中的作用.
主要方法:
- 使用一种基因编码的Ca2+比度传感器,具有低纳米系亲和度和高动态范围.
- 应用传感器的亚细胞准来追踪从血到细胞核的Ca2+动态.
- 研究的信号通路涉及ABA受体,糖糖非发酵1相关蛋白激酶 (SnRK2.2/2.3/2.6) 和Ca2+传感器蛋白激酶 (CPK10/30/32).
主要成果:
- 在各种细胞类型和器官对ABA的反应中可视化独特的时空Ca2+特征.
- 在根盖循环中证明了ABA激活的低纳米Ca2+过渡体和CPK信号.
- 表明盐应激通过ABA信号传递诱导低纳米Ca2+过渡物,抑制根盖成熟和脱落.
结论:
- 发现了一种新型的ABA-Ca2+-CPK信号通路,调节根盖的可塑性.
- 揭示了ABA介导的低纳米Ca2+过渡物,而不是高微分子峰值,在盐应激过程中是关键.
- 通过精确的信号,建立了植物如何适应不利环境的新认识.
相关概念视频
Calmodulin-dependent Signaling
5.1K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.1K
Feedback Regulation of Calcium Concentration
3.3K
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
3.3K
Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
Adaptations that Reduce Water Loss
25.1K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
25.1K
Global Regulatory Systems
2
Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
2
MAPK Signaling Cascades
5.2K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
5.2K


