修改汉克平衡盐溶液作为牙的储存介质:对牙周纤维细胞活性的体外评估
Zhi Jin Lee1, Sook Luan Ng2, Eason Soo1
1Unit of Endodontology and Endodontics, Department of Restorative Dentistry, Faculty of Dentistry, Universiti Kebangsaan Malaysia (UKM), Kuala Lumpur, Malaysia.
概括
将维生素C添加到汉克平衡盐溶液 (HBSS) 中显著改善了牙周带纤维细胞 (PDLF) 活力. 含有维生素C的HBSS提供了一种实用且有效的牙移植存储介质.
科学领域:
- 牙科创伤学 牙科创伤学
- 生物材料科学 生物材料科学
- 细胞生物学 细胞生物学
背景情况:
- 优化牙的存储对于牙周带纤维细胞 (PDLF) 的生存能力至关重要.
- 保存PDLF可以改善牙周纤维的重新连接和牙移植的预后.
- 目前的存储介质需要评估它们在维持细胞活性的有效性.
研究的目的:
- 为了比较各种存储介质在维护PDLF生存能力方面的有效性.
- 为了评估汉克的平衡盐溶液 (HBSS) 和其修改与杜尔贝科的修改介质 (DMEM).
- 为了确定一个最佳的,实用的存储介质的牙.
主要方法:
- 人类牙周组织被用于培养PDLF.
- PDLF细胞暴露于六种介质:HBSS,HBSS + 维生素C,HBSS + PDGF,HBSS + PDGF + 维生素C,HBSS + 血小板酸盐和DMEM.
- 用MTT测定在3,6,12和24小时评估了细胞活力.
主要成果:
- 与HBSS + PDGF和HBSS + PL相比,补充维生素C的介质 (HBSS + Vit C,HBSS + PDGF + Vit C) 保持了显著更高的PDFL活力.
- 所有测试的介质,除了可能平坦的HBSS外,与基线相比,随着时间的推移显示出更高的PDFL活力.
- 虽然DMEM表现出最高的细胞增殖,但对于临床使用来说是不切实际的.
结论:
- 用维生素C补充剂显著提高了HBSS中PDLF活力.
- HBSS + 维生素C 是DMEM的可行和实用的替代品,用于存储损伤的牙.
- 优化的HBSS配方可以改善牙移植手术的结果.
相关概念视频
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01:22Bicarbonate-Carbonic Acid Buffer
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