Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.2K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.2K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

21.8K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
21.8K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

1.8K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Molecular Determinants of Thyroid Cancer Progression: Thyroid Hormone Signaling, the BRAF/MAPK Pathway, and Emerging miRNA Biomarkers.

Biomedicines·2026
Same author

Sirtuin 1 overexpression in mice preserves insulin and thermogenic responses in subcutaneous inguinal white adipose tissue under proinflammatory conditions.

Journal of physiology and biochemistry·2025
Same author

Thyroid Hormones and Co-workers: An Overview.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

A Sensitive Radioimmunoassay for T3 and T4 Determinations in Plasma and Tissues.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Validation of miR-21, miR-31, miR-34A, and miR-203 Changes by Quantitative Polymerase Chain Reaction in Isolated Keratinocytes.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Defective thyroid hormone transport to the brain leads to astroglial alterations.

Neurobiology of disease·2024

相关实验视频

Updated: Jun 6, 2025

Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4
13:02

Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4

Published on: April 7, 2008

36.2K

小鼠胚胎纤维细胞通过T3重编程,诱导多能干细胞.

Ana Montero-Pedrazuela1, Constanza Contreras-Jurado2,3,4

  • 1Instituto de Investigaciones Biomédicas Sols-Morreale, Consejo Superior de Investigaciones Científicas (CSIC), Universidad Autónoma de Madrid (UAM), Madrid, Spain. amontero@iib.uam.es.

Methods in molecular biology (Clifton, N.J.)
|November 23, 2024
PubMed
概括

这项研究详细介绍了一种方法,以改善诱导多能干细胞 (iPSC) 产生,使用三-L-thyronine (T3) 激素. 这种增强的重编程协议可以促进iPSC的产生,并有助于研究再生医学的细胞可塑性.

关键词:
小鼠胚胎纤维细胞.多能性是一种多能性.定量实时PCR可以使用.重编程 重编程 是一种重编程.甲状腺激素激素是甲状腺激素的一种.亚马纳卡的因素 亚马纳卡的因素iPSC 殖民地形成的形成.

更多相关视频

Generation of Induced Pluripotent Stem Cells by Reprogramming Mouse Embryonic Fibroblasts with a Four Transcription Factor, Doxycycline Inducible Lentiviral Transduction System
11:48

Generation of Induced Pluripotent Stem Cells by Reprogramming Mouse Embryonic Fibroblasts with a Four Transcription Factor, Doxycycline Inducible Lentiviral Transduction System

Published on: November 13, 2009

24.6K
Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
11:00

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

Published on: December 16, 2016

7.1K

相关实验视频

Last Updated: Jun 6, 2025

Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4
13:02

Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4

Published on: April 7, 2008

36.2K
Generation of Induced Pluripotent Stem Cells by Reprogramming Mouse Embryonic Fibroblasts with a Four Transcription Factor, Doxycycline Inducible Lentiviral Transduction System
11:48

Generation of Induced Pluripotent Stem Cells by Reprogramming Mouse Embryonic Fibroblasts with a Four Transcription Factor, Doxycycline Inducible Lentiviral Transduction System

Published on: November 13, 2009

24.6K
Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
11:00

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

Published on: December 16, 2016

7.1K

科学领域:

  • 干细胞生物学 干细胞生物学
  • 分子生物学分子生物学
  • 内分泌学 在内分泌学.

背景情况:

  • 身体细胞可以通过使用特定的转录因子 (Yamanaka因子) 重编程成诱导多能干细胞 (iPSC).
  • 优化重编程效率对于推进再生医学和疾病建模至关重要.

研究的目的:

  • 提出一种使用triiodo-L-thyronine (T3) 来增强来自小鼠胚胎纤维细胞 (MEF) 的诱导多能干细胞 (iPSC) 生成的方案.
  • 描述分析iPSC多能性的方法,包括性酸酶染色和基因表达造型.

主要方法:

  • 使用Yamanaka因子的逆转录病毒传递来重新编程MEF,并添加了triiodo-L-thyronine (T3).
  • 对性酸酶活性进行殖民地染色,以识别多能干细胞.
  • 定量实时PCR (qPCR) 用于分析扩大的iPSC殖民地内源性多能性基因表达.

主要成果:

  • 三多-L-thyronine (T3) 补充剂可以提高诱导多能干细胞 (iPSC) 生产的效率.
  • 已建立的iPSC殖民地表现出性酸酶活性,证实多能性.
  • 基因表达分析证实了T3增强iPSC中关键多能性标记物的内源表达.

结论:

  • 将triiodo-L-thyronine (T3) 集成到重编程协议中,可以显著改善诱导多能干细胞 (iPSC) 生产.
  • 这种增强的方法促进了功能性iPSCs的生成,支持细胞可塑性,疾病建模和再生疗法的研究.